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Journal of Biosciences

, Volume 4, Issue 4, pp 431–439 | Cite as

Regulation of phosphoglycerate mutase in developing forespores and dormant spores ofBacillus megaterium by thein vivo levels of phosphoglycerate mutase inhibitor

  • Ravendra Pal Singh
Article

Abstract

Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75–85%) during spore germination. No appreciable amount of the inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis.

Keywords

Bacillus megaterium 3-phosphoglyceric acid phosphoglycerate mutase mutase inhibitor dipicolinic acid 

Abbreviations used

3-PGA

3-phosphoglyceric acid

2-PGA

2-phosphoglyceric acid

DPA

dipicolinic acid

SNB

supplemented nutrient broth

DEAE

diethylamino ethyl

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Copyright information

© Indian Academy of Sciences 1982

Authors and Affiliations

  • Ravendra Pal Singh
    • 1
  1. 1.Department of BiochemistryUniversity of Connecticut, Health CenterFarmingtonUSA

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