Abstract
Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75–85%) during spore germination. No appreciable amount of the inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis.
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Abbreviations
- 3-PGA:
-
3-phosphoglyceric acid
- 2-PGA:
-
2-phosphoglyceric acid
- DPA:
-
dipicolinic acid
- SNB:
-
supplemented nutrient broth
- DEAE:
-
diethylamino ethyl
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Singh, R.P. Regulation of phosphoglycerate mutase in developing forespores and dormant spores ofBacillus megaterium by thein vivo levels of phosphoglycerate mutase inhibitor. J Biosci 4, 431–439 (1982). https://doi.org/10.1007/BF02704636
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DOI: https://doi.org/10.1007/BF02704636