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Journal of Biosciences

, Volume 6, Issue 4, pp 525–533 | Cite as

Genetic transformation in bacteria

  • N. K. Notani
  • V. P. Joshi
  • R. P. Kanade
Article
  • 394 Downloads

Abstract

Certain species of bacteria can become competent to take up high molecular weight DNA from the surrounding medium. DNA homologous to resident chromosomal DNA is transported, processed and recombined with the resident DNA. There are some variations in steps leading to transformation between Gram-positive bacteria likebiplococcus pneumoniae and Gram-negative bacteria represented byHaemophilus influenzae but the integration is by single-strand displacement in both cases. Plasmid (RSF0885) transformation is low inHaemophilus influenzae but this is increased significantly if (homologous) chromosomal DNA is spliced to plasmid DNA. InHaemophilus influenzae, rec1 function is required for peak transformation with chimeric plasmids. Chimeric plasmid fixed presumably extrachromosomally undergoes frequent recombination between homologous segments contained in resident chromosome and the plasmid.

Keywords

DNA-cell interaction competence specificity in DNA uptake single-strand displacement chimeric plasmid transformation rec1 function recombination 

Abbreviations used

Mr

Molecular weight

CF

competence factor

BF

binding factor

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Copyright information

© Indian Academy of Sciences 1984

Authors and Affiliations

  • N. K. Notani
    • 1
  • V. P. Joshi
    • 1
  • R. P. Kanade
    • 1
  1. 1.Biology and Agriculture DivisionBhabha Atomic Research CentreBombayIndia

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