Journal of Biosciences

, Volume 21, Issue 4, pp 487–496 | Cite as

Biodegradation of commercial linear alkyl benzenes byNocardia amarae

  • Manju Bhatia
  • H. Devendra Singh


Laboratory degradation studies of two indigeneously produced linear alkyl benzenes byNocardia amarae MB-11 isolated from soil showed an overall degradation of linear alkyl benzenes isomers to the extent of 57–70%. Degradation of 2-phenyl isomers of linear alkyl benzenes was complete and faster than that of other phenyl position (C3–C7) isomers which were degraded to the extent of 40–72% only. Length of alkyl side chains (C10–C14) had little or no impact on the degradation pattern. Major metabolities detected were 2-, 3-and 4-phenyl butyric acids, phenyl acetic acid and cis, cis-muconic acid. Minor metabolites weretrans-cinnamic acid, 4-phenyl 3-butenoic acid and 3-phenyl pentanoic acid along with two unidentified hydroxy acids. On the basis of the formation pattern of these metabolities, three catabolic pathways of linear alkyl benzenes isomers inNocardia amarae MB-11 were postulated. All the phenyl position (C2–C7) isomers of C10, C12, and C14 linear alkyl benzenes along with 3-phenyl and 5-phenyl isomers of C11 and C13 linear alkyl benzenes were degraded viacis,cis-muconic acid pathway. Other phenyl position isomers of C11 and C13 linear alkyl benzenes with phenyl substitution at even number carbon atoms were principally degraded via phenyl acetic acid pathway whiletrans-cinnamic acid formation provided a minor pathway


Linear alkyl benzenes Nocardia amarae degradation patterns catabolic pathways 


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  1. Amund O O and Higgins I J 1985 The degradation of 1-phenylalkanes by an oil degrading strain ofAcinetobacter lwoffi;Antonie van Leeuwenhoek: J. Microbiol. Serol. 51 45–56CrossRefGoogle Scholar
  2. Bayona J M, Albaiges J, Solanas A M and Grifoll M 1986 Selective aerobic degradation of LAB by pure microbial cultures;Chemosphere 15 595–598CrossRefGoogle Scholar
  3. Davis J B and Raymond RL 1961 Oxidation of alkyl substituted hydrocarbons by aNocardia during growth on n-alkanes;Appl. Microbial. 9 383–388Google Scholar
  4. Eganhouse R P 1986 Long chain alkylbenzenes: their analytical chemistry, environmental occurrence and fate;Int. J. Environ. Anal. Chem. 26 241–263PubMedCrossRefGoogle Scholar
  5. Eganhouse R P, Blumfield D L and Kaplan I R 1983 Long chain alkylbenzenes as molecular tracers of domestic waste in the marine environment;Environ. Sci. Technol. 17 523–530CrossRefGoogle Scholar
  6. Gledhill W E 1974 LAS: Biodegradation and aquatic interactions;Adv. Appl. Microbial. 17 265–293CrossRefGoogle Scholar
  7. Ishiwatari R, Takada H, Yun S-J and Matsumoto E 1983 Alkylbenzene pollution in Tokyo Bay Sediments;Nature (London) 301 599–600CrossRefGoogle Scholar
  8. Metcalfe L D and Schmitz A A 1961 The rapid preparation of fatty acid esters for gas chromatographic analysis;Anal. Chem. 33 363–364CrossRefGoogle Scholar
  9. Raymond R L, Jamison V W and Hudson J O 1967 Microbial hydrocarbon co-oxidation I. Oxidation of monoand dicyclic hydrocarbons by soil isolates of the genusNocardia;Appl. Microbiol. 15 857–865PubMedGoogle Scholar
  10. Reddy P G, Singh H D, Pathak M G, Bhagat S D and Baruah J N 1983 Isolation and functional characterisation of hydrocarbon emulsifying and solubilising factors produced by aPseudomonas species;Biotechnol. Bioeng. 25 387–401CrossRefPubMedGoogle Scholar
  11. Sariaslani F S, Harper D B and Higgins I J 1974 Microbial biodegradation of hydrocarbons: Catabolism of 1-phenyl alkanes byNocardia salmonicolor;Biochemn. J. 140 31–45Google Scholar
  12. Swisher R D 1963 Biodegradation of ABS in relation to chemical structure;J. Water Pollut. Cont. Fed. 35 877–892Google Scholar
  13. Swisher R D 1987Surfactant Biodegradation (New York: Marcel Dekker)Google Scholar
  14. Walsh C 1978Enzymatic reaction mechanisms (San Francisco: W H Freeman) p 701Google Scholar
  15. Webley D M, Duff R B and Farmer V C 1956 Evidence for β-oxidation in the metabolism of saturated aliphatic hydrocarbons by soil species ofNocardia;Nature (London) 178 1467–1468CrossRefGoogle Scholar
  16. Willets A J 1973 Microbial aspects of the biodegradation of synthetic detergents: A reviewInt. Biodem. Bull. 9 3–10Google Scholar

Copyright information

© Indian Academy of Sciences 1996

Authors and Affiliations

  • Manju Bhatia
    • 1
  • H. Devendra Singh
    • 1
  1. 1.Department of BiochemistryManipur University, ImphalManipurIndia

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