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Journal of Biosciences

, Volume 14, Issue 2, pp 153–162 | Cite as

Qualitative and quantitative variation of tRNA in certain invertebrates

  • P. Sivaram
  • R. Mayasundari
Article
  • 27 Downloads

Abstract

Total tRNA was isolated, purified and quantitated from earthworm, cockroach, fresh water mussel and rat liver. The total tRNA content of invertebrates was found to be much lower than that of rat liver. When checked for aminoacylation capacity with homologous and heterologous enzymes and algal protein hydrolysate, the tRNA preparation from rat liver and fresh water mussel, a mollusc, were found to be active. On the other hand, the tRNAs from earthworm, an annelid, and cockroach, an arthropod, were completely inactive with the homologous enzymes but showed partial activity with heterologous enzymes. Similar results were obtained with individual amino acids also. The low activity or inactivity of earthworm and cockroach tRNAs appears to be due to certain endogenous aminoacylation inhibitors.

Keywords

Transfer RNA aminoacyl-tRNA synthetase aminoacylation earthworm cockroach fresh water mussel 

Abbreviations used

BSA

Borine serum albumin

DTT

dithiothreitol

TCA

trichloroacetic acid

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Copyright information

© Indian Academy of Sciences 1989

Authors and Affiliations

  • P. Sivaram
    • 1
  • R. Mayasundari
    • 1
  1. 1.Department of GeneticsPost-Graduate Institute of Basic Medical SciencesMadrasIndia

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