Journal of Biosciences

, Volume 12, Issue 3, pp 257–265 | Cite as

Effect of steroid hormones on vaginal epithelial cells: Anin vitro model for steroid hormone action

  • S. Vijayasaradhi
  • A. Khar
  • P. D. Gupta


Conditions have been standardized to maintain rat vaginal epithelial cellsin vitro with more than 95% viability. Cultured epithelial cells were used to study the effects of normal fetal calf scrum, estradiol and progesterone on the incorporation of [3H]-uridine in RNA and incorporation of [14C]-aminoacids in proteins. While fetal calf serum and estradiol stimulate the incorporation of both uridine and afno acids, progesterone did not show any effect. Estradiol treated vaginal cells show typical fcroridges (indicative of keratinization of cells) in contrast to estradiol deprived cells, which show microvilli on cell surface when examined in scanning electron microscope.


Steroid hormones vaginal epithelial cells fetal calf serum 

Abbreviations used


Phosphate buffered saline


fnimal essential medium


Dulbecco’s modified eagles medium: Ham’s F-12


fetal calf serum


trichloroacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen, T. C. and Leavitt, W. W. (1981)J. Steroid Biochem.,14, 29.PubMedCrossRefGoogle Scholar
  2. Armeiin, H. A., Wishikawa, K. and Sato, G. H. (1974) inControl of Proliferation in Animal Cells (eds B. Clarkson and R. Baserga) (New York: Cold Spring Harbor) p. 97.Google Scholar
  3. Brennan, J. K., Mansky, J., Roberts, G. and Lichtman, M. A. (1975)In vitro,11, 354.PubMedCrossRefGoogle Scholar
  4. Brock, M. L and Shapiro, D. J. (1983)Cell,34, 207.PubMedCrossRefGoogle Scholar
  5. Conti, C. J. and Tasat, D. R. (1986)J. Steroid Biochem.,24, 747.PubMedCrossRefGoogle Scholar
  6. Flaxman, B. A, Chopra, D. P. and Newman, D. (1974)In vitro,9, 194.CrossRefGoogle Scholar
  7. Gupta, P. D., Khar, A. and Vijayasaradhi, S. (1986)Indian J. Exp. Biol.,24, 679.PubMedGoogle Scholar
  8. Hennings, H., Holbrook, K., Steinert, P. and Yuspa, S. H. (1980) inThe Biochemistry of Normal and Abnormal Skin (eds I. A. Bernstein and M. Seiji) (Tokyo: University of Tokyo Press) p. 3.Google Scholar
  9. Iguchi, T., Uchima, F. D. A., Ostrande, P. L. and Bern, H. A. (1983)Proc. Natl. Acad. Sci. USA,80, 3743.PubMedCrossRefGoogle Scholar
  10. Jensen, E. V. and DeSombre, E. R. (1972)Annu. Rev. Biochem.,41, 203.PubMedCrossRefGoogle Scholar
  11. Katzmam, P. A., Larson, D. L. and Podratz, K. C. (1971) inSex Steroids (ed. K. W. McKeens) (New York: Appleton-Century-Crofts) p. 107.Google Scholar
  12. Kronenberg, M. S. and Clark, J. H. (1985)Endocrinology,117, 1480.PubMedCrossRefGoogle Scholar
  13. Leroy, F., Galand, P. and Chretein, J. (1969)J. Endocrinol.,45, 441.PubMedCrossRefGoogle Scholar
  14. Morley, C. G. D. and Kingdon, H. S. (1972)Anal. Biochem.,45, 298.PubMedCrossRefGoogle Scholar
  15. Parakkal, P. F. (1974)Anat. Rec.,178, 529.PubMedCrossRefGoogle Scholar
  16. Parakkal, P. F. and Gregoire, A. T. (1972)Biol. Reprod.,6, 117.PubMedGoogle Scholar
  17. Vijayasaradhi, S. and Gupta, P, D. (1987)J. Submicrosc. Cytol., (in press).Google Scholar
  18. Yang, J., Richards, J., Bowman, P., Guzman, R., Enami, J., McCormic, K., Hamamoto, S., Pitelka, D. and Nandi, S. (1979)Proc. Natl. Acad. Sci., USA,76, 3401.PubMedCrossRefGoogle Scholar

Copyright information

©  Indian Academy of Sciences 1987

Authors and Affiliations

  • S. Vijayasaradhi
    • 1
  • A. Khar
    • 1
  • P. D. Gupta
    • 1
  1. 1.Centre for Cellular and Molecular BiologyHyderabadIndia

Personalised recommendations