Abstract
Conditions have been standardized to maintain rat vaginal epithelial cellsin vitro with more than 95% viability. Cultured epithelial cells were used to study the effects of normal fetal calf scrum, estradiol and progesterone on the incorporation of [3H]-uridine in RNA and incorporation of [14C]-aminoacids in proteins. While fetal calf serum and estradiol stimulate the incorporation of both uridine and afno acids, progesterone did not show any effect. Estradiol treated vaginal cells show typical fcroridges (indicative of keratinization of cells) in contrast to estradiol deprived cells, which show microvilli on cell surface when examined in scanning electron microscope.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PBS:
-
Phosphate buffered saline
- MEM:
-
fnimal essential medium
- DME-F12:
-
Dulbecco’s modified eagles medium: Ham’s F-12
- FCS:
-
fetal calf serum
- TCA:
-
trichloroacetic acid
References
Allen, T. C. and Leavitt, W. W. (1981)J. Steroid Biochem.,14, 29.
Armeiin, H. A., Wishikawa, K. and Sato, G. H. (1974) inControl of Proliferation in Animal Cells (eds B. Clarkson and R. Baserga) (New York: Cold Spring Harbor) p. 97.
Brennan, J. K., Mansky, J., Roberts, G. and Lichtman, M. A. (1975)In vitro,11, 354.
Brock, M. L and Shapiro, D. J. (1983)Cell,34, 207.
Conti, C. J. and Tasat, D. R. (1986)J. Steroid Biochem.,24, 747.
Flaxman, B. A, Chopra, D. P. and Newman, D. (1974)In vitro,9, 194.
Gupta, P. D., Khar, A. and Vijayasaradhi, S. (1986)Indian J. Exp. Biol.,24, 679.
Hennings, H., Holbrook, K., Steinert, P. and Yuspa, S. H. (1980) inThe Biochemistry of Normal and Abnormal Skin (eds I. A. Bernstein and M. Seiji) (Tokyo: University of Tokyo Press) p. 3.
Iguchi, T., Uchima, F. D. A., Ostrande, P. L. and Bern, H. A. (1983)Proc. Natl. Acad. Sci. USA,80, 3743.
Jensen, E. V. and DeSombre, E. R. (1972)Annu. Rev. Biochem.,41, 203.
Katzmam, P. A., Larson, D. L. and Podratz, K. C. (1971) inSex Steroids (ed. K. W. McKeens) (New York: Appleton-Century-Crofts) p. 107.
Kronenberg, M. S. and Clark, J. H. (1985)Endocrinology,117, 1480.
Leroy, F., Galand, P. and Chretein, J. (1969)J. Endocrinol.,45, 441.
Morley, C. G. D. and Kingdon, H. S. (1972)Anal. Biochem.,45, 298.
Parakkal, P. F. (1974)Anat. Rec.,178, 529.
Parakkal, P. F. and Gregoire, A. T. (1972)Biol. Reprod.,6, 117.
Vijayasaradhi, S. and Gupta, P, D. (1987)J. Submicrosc. Cytol., (in press).
Yang, J., Richards, J., Bowman, P., Guzman, R., Enami, J., McCormic, K., Hamamoto, S., Pitelka, D. and Nandi, S. (1979)Proc. Natl. Acad. Sci., USA,76, 3401.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vijayasaradhi, S., Khar, A. & Gupta, P.D. Effect of steroid hormones on vaginal epithelial cells: Anin vitro model for steroid hormone action. J Biosci 12, 257–265 (1987). https://doi.org/10.1007/BF02703070
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02703070