Journal of Biosciences

, Volume 18, Issue 1, pp 1–11 | Cite as

Promoter search and strength of a promoter: two important means for regulation of gene expression inEscherichia coli

  • Rakesh K. Mishra
  • Dipankar Chatterji


Search for a promoter element by RNA polymerase from the extremely large DNA base sequence is thought to be the slowest and rate-determining for the regulation of transcription process. Few direct experiments we described here which have tried to follow the mechanistic implications of this promoter search. However, once the promoter is located, transcription complex, constituting mainly the RNA polymerase molecule and few transcription factors has to unidirectionally clear the promoter and elongate the RNA chain through a series of steps which altogether define the initiation of transcription process. Thus, it appears that the promoter sequence acts as a trap for RNA polymerase associated with a large binding constant, although to clear the promoter and to elongate the transcript such energy barrier has to be overcome. Topological state of the DNA, particularly in the neighbourhood of the promoter plays an important role in the energetics of the whole process


Escherichia coli promoters promoter strength abortive cycling sigma cycle 


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Copyright information

© Indian Academy of Sciences 1993

Authors and Affiliations

  • Rakesh K. Mishra
    • 1
  • Dipankar Chatterji
    • 1
  1. 1.Centre for Cellular and Molecular BiologyHyderabadIndia

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