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Journal of Biosciences

, Volume 5, Supplement 1, pp 149–155 | Cite as

Towards a subunit influenza vaccine prepared by affinity chromatography on immobilized lectin

  • T. Kristiansen
  • M. Sparrman
  • L. Heller
Article

Abstract

Disintegration of influenza virions with 0.2% w/v sodium deoxycholate releases haemagglutinin and neuraminidase, which in the presence of detergent are both adsorbed to the lectin fromCrotalaria juncea, coupled to Sepharose 2B.

The binding is mediated through galactosyl residues on haemagglutinin and neural-minidase, which are completely displaced in 0.2 M lactose and co-elute in a narrow zone.

The immunogenicity of haemagglutinin and neuraminidase in mice is markedly increased after adsorption onto lipid, particles constituting “intralipid” (Kabi Vitrum, Stocholm, Sweden).

Keywords

Influenza subunit vaccine haemagglutinin neuraminidase lectin Crotalaria juncea affinity chromatography adjuvant 

Abbreviations used

HA

Haemagglutinin

NA

neuraminidase

Dol

Dolichol

Asn

asparagine

Ser

serine

Man

mannose

Glc

glucose

Glc N-Ac

glucose N-acetylamine

Thr

threonine

Mr

molecular weight

TNBP

tri-n-butylphosphate

CTAB

Cetyl trimethyl ammonium bromide

SDOC

sodium deoxycholate

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Copyright information

© Indian Academy of Sciences 1983

Authors and Affiliations

  • T. Kristiansen
    • 1
  • M. Sparrman
    • 1
  • L. Heller
    • 1
    • 2
  1. 1.Institute of Biochemistry, Biomedical CenterUniversity of UppsalaUppsalaSweden
  2. 2.National Bacteriological LaboratoryStockholmSweden

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