Journal of Biosciences

, Volume 5, Supplement 1, pp 65–71 | Cite as

Lectins fromGriffonia simplicifolia seeds

  • Irwin J. Goldstein


The physical-chemical and carbohydrate binding specificity ofGriffonia simplicifolia I (GS I) isolectins, one of the 4 lectins isolated fromGriffonia simplicifolia seeds, are described.

Association constants for the binding of methyl α- and β-D-galactopyranoside and methyl 2-acetamido-2-deoxy-α-D-galactopyranoside to the A4, A2 B2 and B4 isolectins are reported.

Precipitation reactions of theGriffonia simplicifolia isolectins with guaran and type B blood group substance are described.

The hypothesis that subunit B is a precursor of subunit A, a process involving proteolytic cleavage of the B subunit, was tested by conducting structural studies on the 2 subunits. The results indicated that the A and B subunits are probably products of 2 separate but closely related, possibly contiguous genes.


Lectin Bandeiraea simplicifolia Griffonia simplicifolia isolectins carbohydrate binding 

Abbreviations used


A system of 5α-D-galactosyl binding isolectins


a N-acetyl-D-glucosaminyl binding lectin, GS III, a system of 5,2-acetamido-2-deoxy-α-D-galactosyl binding lectins


a lectin with Leb (Lewis-b) binding properties, all lectins isolated fromG. simplicifolia seeds


polyacrylamide gel electrophoresis


sodium dodecylsulphate PAGE


bovine serum alubmin

p-NO2 - ph



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  1. DeBoeck, H., Loontiens, F. G., Delmotte, F. M. and DeBruyne, C. D. (1981)FEBS Lett.,126, 227.CrossRefGoogle Scholar
  2. Delmotte, F. M. and Goldstein, I. J. (1980)Eur. J, Biochem.,112, 219.CrossRefGoogle Scholar
  3. Eckhardt, A. E. and Goldstein, I. J. (1979)Glyconjugate Res.,11, 1043.Google Scholar
  4. Goldstein, I. J., Blake, D. A., Ebisu, S., Williams, T. J. and Murphy, L. A. (1981)J. Biol. Chem.,256, 3890.PubMedGoogle Scholar
  5. Hayes, C.E. and Goldstein, I. J. (1974)J. Biol Chem.,249, 1904.PubMedGoogle Scholar
  6. HoŘejši, V. and Kocourek, J. (1973)Biochim. Biophys. Acta,297, 346.PubMedGoogle Scholar
  7. Lamb, J. E., Bookstein, F. L., Goldstein, I. J. and Newton, L. E. (1981)J. Biol. Chem.,256, 5874.PubMedGoogle Scholar
  8. Lonngren, J., Goldstein, I. J. and Zand, R. (1976)Biochemistry,15, 436.PubMedCrossRefGoogle Scholar
  9. MÄkelÄ, O. and MÄkelÄ, P. (1956)Ann. Med. Exp. Biol. Fenn.,34, 403.Google Scholar
  10. Murphy, L. A. and Goldstein, I. J. (1977)J. Biol. Chem.,252, 4739.PubMedGoogle Scholar
  11. Murphy, L. A. and Goldstein, I. J. (1979)Biochemistry,18, 4999.PubMedCrossRefGoogle Scholar
  12. Peters, B.P. and Goldstein, I. J. (1979)Exp. Cell Res.,120, 321.PubMedCrossRefGoogle Scholar
  13. Ross, T. T., Hayes, C. E. and Goldstein, I. J. (1976)Carbohydr. Res.,47, 91.PubMedCrossRefGoogle Scholar
  14. Stanley, W. S., Peters, B. P., Blake, D. A., Yep, D., Chu, E. H. Y. and Goldstein, I.J. (1979)Proc. Natl. Acad. Sci. USA,76, 304.Google Scholar
  15. Wood, C, Kabat, E. A., Murphy, L. A. and Goldstein, I. J. (1979)Arch. Biochem. Biophys.,198, 1.PubMedCrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 1983

Authors and Affiliations

  • Irwin J. Goldstein
    • 1
  1. 1.Department of Biological ChemistryUniversity of MichiganAnn ArborUSA

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