The physical-chemical and carbohydrate binding specificity ofGriffonia simplicifolia I (GS I) isolectins, one of the 4 lectins isolated fromGriffonia simplicifolia seeds, are described.
Association constants for the binding of methyl α- and β-D-galactopyranoside and methyl 2-acetamido-2-deoxy-α-D-galactopyranoside to the A4, A2 B2 and B4 isolectins are reported.
Precipitation reactions of theGriffonia simplicifolia isolectins with guaran and type B blood group substance are described.
The hypothesis that subunit B is a precursor of subunit A, a process involving proteolytic cleavage of the B subunit, was tested by conducting structural studies on the 2 subunits. The results indicated that the A and B subunits are probably products of 2 separate but closely related, possibly contiguous genes.
KeywordsLectin Bandeiraea simplicifolia Griffonia simplicifolia isolectins carbohydrate binding
- GS I
A system of 5α-D-galactosyl binding isolectins
- GS II
a N-acetyl-D-glucosaminyl binding lectin, GS III, a system of 5,2-acetamido-2-deoxy-α-D-galactosyl binding lectins
- GS IV
a lectin with Leb (Lewis-b) binding properties, all lectins isolated fromG. simplicifolia seeds
polyacrylamide gel electrophoresis
sodium dodecylsulphate PAGE
bovine serum alubmin
- p-NO2 - ph
Unable to display preview. Download preview PDF.
- Eckhardt, A. E. and Goldstein, I. J. (1979)Glyconjugate Res.,11, 1043.Google Scholar
- MÄkelÄ, O. and MÄkelÄ, P. (1956)Ann. Med. Exp. Biol. Fenn.,34, 403.Google Scholar
- Stanley, W. S., Peters, B. P., Blake, D. A., Yep, D., Chu, E. H. Y. and Goldstein, I.J. (1979)Proc. Natl. Acad. Sci. USA,76, 304.Google Scholar