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Journal of Biosciences

, Volume 5, Supplement 1, pp 65–71 | Cite as

Lectins fromGriffonia simplicifolia seeds

  • Irwin J. Goldstein
Article

Abstract

The physical-chemical and carbohydrate binding specificity ofGriffonia simplicifolia I (GS I) isolectins, one of the 4 lectins isolated fromGriffonia simplicifolia seeds, are described.

Association constants for the binding of methyl α- and β-D-galactopyranoside and methyl 2-acetamido-2-deoxy-α-D-galactopyranoside to the A4, A2 B2 and B4 isolectins are reported.

Precipitation reactions of theGriffonia simplicifolia isolectins with guaran and type B blood group substance are described.

The hypothesis that subunit B is a precursor of subunit A, a process involving proteolytic cleavage of the B subunit, was tested by conducting structural studies on the 2 subunits. The results indicated that the A and B subunits are probably products of 2 separate but closely related, possibly contiguous genes.

Keywords

Lectin Bandeiraea simplicifolia Griffonia simplicifolia isolectins carbohydrate binding 

Abbreviations used

GS I

A system of 5α-D-galactosyl binding isolectins

GS II

a N-acetyl-D-glucosaminyl binding lectin, GS III, a system of 5,2-acetamido-2-deoxy-α-D-galactosyl binding lectins

GS IV

a lectin with Leb (Lewis-b) binding properties, all lectins isolated fromG. simplicifolia seeds

PAGE

polyacrylamide gel electrophoresis

SDS-AGE

sodium dodecylsulphate PAGE

BSA

bovine serum alubmin

p-NO2 - ph

p-nitrophenyl

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Copyright information

© Indian Academy of Sciences 1983

Authors and Affiliations

  • Irwin J. Goldstein
    • 1
  1. 1.Department of Biological ChemistryUniversity of MichiganAnn ArborUSA

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