Abstract
Both the high molecular weight and the low molecular weight variants of urinary Y-glutamyl transpeptidase, displayed transpeptidase (pH optimum 8.6) and autotrans-peptidase (pH optimum 9.4) activities. Iodoacetamide inhibited the transpeptidase activity more efficiently than the autotranspeptidase activity with respect to both variants of Y-glutamyl transpeptidase. The high molecular weight form utilized L-glutamine as a better acceptor than L-cystine during the transpeptidation reaction whereas the reverse was the case with the low molecular weight variant. While phenylmethylsulphonyl fluoride-treated enzymes retained full activitiesper se, addition of maleic acid to the modified enzyme was found to inhibit the catalytic activities indicating a maleic acid-induced conformational change of the modified enzyme.
Similar content being viewed by others
Abbreviations
- TNBS:
-
trinitrobenzene sulphonic acid
- PMSF:
-
phenylmethylsulphonyl fluoride
- DTNB:
-
dithiobis 2-nitrobenzoic acid
- IA:
-
iodoacetamide
- Gly:
-
glycine
- L-Leu:
-
leucine
- L-Ala:
-
alanine
- L-Cyst:
-
cysteine
- L-Glu:
-
L-glutamine
References
Elce, J. S. (1978) inFunctions of glutathione in liver and kidney, eds. H. Sies and A. Wendel (Berlin: Springer Verlag) p. 78.
Elce, J. S. (1980)Biochem. J. 185, 473.
Fields, R. (1972)Methods Enzymol.,25, 464.
Gurd, F. R. N. (1972)Methods Enzymol.,25, 424.
Habee, A. F. S. A. (1972)Methods Enzymol.,25, 457.
Inoue, M., Horiuchi, S. and Morino, Y. (1977)Eur.J. Biochem.,73, 335.
Inoue, M., Horiuchi, S. and Morino,Y. (1978)Biochem. Biophys. Res. Commun.,82, 1183
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R.J. (1951)J. Biol. Chem.,193, 265.
Miller, S. P., Awasthi, Y. C. and Srivastava, S. K. (1976)J.Biol. Chem.,251, 2271
Orlowski, M. and Meister, A. (1965)J. Biol. Chem.,240, 338.
Parsons, S. M. Jao, L., Dahl Quist, F.W., Bordes, C. L., Groff, T., Races, J. and Raftery, M. A. (1969)Biochemistry,8, 700.
Rambabu, K. and Pattabiraman, T. N. (1976)Clin. Chim. Acta,73, 251.
Rambabu, K., Jacob, R. T. and Pattabiraman, T. N. (1981)Clin. Chim. Acta,109, 257.
Rosalki, S. B. (1975) inAdvancesin clin ical chemistry, eds O. Bodansky and A.L. Latner (New York: Academic Press) p. 53.
Shaw, L. M, London, J. W., Fetterolf, D. and Garfinkel, D. (1977)Clin. Chem.,23, 79.
Shaw, L. M., London, J. W. and Peterson, L. E. (1978)Clin. Chem.,24, 905
Tate, S. S. and Meister, A. (1974)Proc. Natl. Acad. Sci. USA,71, 3329.
Tate, S. S. and Ross, E. M. (1977).Biol. Chem.,252, 6042
Tate, S. S. and Meister, A. (1978)Proc. Natl. Acad. Sci. USA,75, 4806.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rambabu, K., Pattabiraman, T.N. Studies on the properties of the variants of gamma-glutamyl transpeptidase in human urine. J. Biosci. 4, 287–294 (1982). https://doi.org/10.1007/BF02702741
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02702741