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Journal of Biosciences

, Volume 4, Issue 2, pp 191–195 | Cite as

Kinetic characterization of rat liver nuclear lysozyme

  • V. Sidhan
  • S. Gurnani
Article

Abstract

The kinetic properties of rat liver nuclear lysozyme, earlier purified to homogeneity in our laboratory, have been studied. The enzyme was found to be maximally active in the pH range 4.2 to 5.4 in 0.02 M buffer. Its Km was found to be 333 mg/litre. It was heat sensitive even in the acidic pH range. The enzyme exhibited tissue specific differences when compared with the rat kidney nuclear lysozyme.

Keywords

Nuclear lysozyme characterization tissue specific differences pH optimum Km value 

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Copyright information

© Indian Academy of Sciences 1982

Authors and Affiliations

  • V. Sidhan
    • 1
  • S. Gurnani
    • 1
  1. 1.Biochemistry and Food Technology DivisionBhabha Atomic Research CentreBombay

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