Advertisement

Journal of Biosciences

, Volume 9, Issue 3–4, pp 129–135 | Cite as

Strontium-calcium discriminationin vitro by rat tissues

  • S. G. Kshirsagar
Article

Abstract

Uptake of89Sr and45Ca by 15 soft tissues of adult rat was studiedin vitro to assess the extent of discrimination between Sr and Ca. While brain, kidney, placenta and uterus have lower uptake of89Sr and 45 Ca that of diaphragm, lactating mammary gland, skeletal muscle, skin, spleen and testes is higher. Tissues with medium range uptake are heart, small intestine, liver, lung, non-lactating mammary gland and ovary. The 6 tissues displaying discriminating ability, as expressed by89Sr/45Ca (tissue/medium), in the decreasing order are: small intestine, kidney, lactating mammary gland, placenta, diaphragm and heart. Non-lactating mammary gland and the other tissues did not differentiate between Sr and Ca. The efect of several enzyme inhibitors, compounds influencing Sr-Ca metabolism and other factors was studied in terms of the nature and mechanism of Sr-Ca discrimination.

Keywords

Sr/Ca discriminationin vitro rat tissues 

Abbreviations used

DNP

2-4-Dinitrophenol

OR

observed ratio

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Comar, C. L. (1955)Radioisotopes in biology and agriculture (New York: McGraw Hill) p. 191, 303Google Scholar
  2. Comar, C. L. (1963) inThe transfer of calcium and strontium across the biological membranes (ed. R. H. Wasserman) (New York, London: Academic Press) p. 405.Google Scholar
  3. Comar, C. L. and Wasserman, R. H. (1964) inThe mineralmetabolism–an advanced treatise (eds C. L. Comar and F. Bronner) (London, New York: Academic Press) Vol. 2, Part A, p. 523.Google Scholar
  4. Comar, C. L., Wasserman, R. H. and Nold, M. M. (1956)Proc. Soc. Exp. Biol. Med.,92, 859.PubMedGoogle Scholar
  5. Catsch, A. (1957)Naturwissenschaften,44, 94.CrossRefGoogle Scholar
  6. Della Rosa, R. J., Smith, F. A. and Stannard, J. N. (1961)Int. J. Radiat. Biol.,3, 557.PubMedCrossRefGoogle Scholar
  7. Ebashi, S. and Lipmann, F. (1962)J. Cell. Biol.,14, 389.CrossRefGoogle Scholar
  8. Kimberg, D. V., Schachter, D. and Schenker, H. (1961)Am. J. Physiol.,200, 1256.PubMedGoogle Scholar
  9. Kshirsagar, S. G. (1976a)J. Nutr.,106, 1475.PubMedGoogle Scholar
  10. Kshirsagar, S. G. (1976b)Indian J. Exp. Biol.,14, 424.PubMedGoogle Scholar
  11. Kshirsagar, S. G. (1977a)Int. J. Radiat. Biol.,32, 561.CrossRefGoogle Scholar
  12. Kshirsagar, S. G. (1977b)Indian J. Exp. Biol.,15, 447.PubMedGoogle Scholar
  13. Kshirsagar, S. G., Lloyd, E. and Vaughan, J. (1966)Br. J. Radiol.,39, 131.CrossRefGoogle Scholar
  14. Lengemann, F. W. (1960)J. Biol. Chem.,233, 1859.Google Scholar
  15. Menczel, J. and Mor, E. (1972) inSecond International Conference on Strontium Metobolism, USAEC report, Conf720818, p. 273.Google Scholar
  16. Martonosi, A., Pucell, A. G. and Halpin, R. A. (1971) inCellular mechanisms for calcium transfer and homeostasis (eds G. Nichols Jr and R. H. Wasserman) (New York, London: Academic Press) p. 175.Google Scholar
  17. Olson, E. J. and Cazart, R. J. (1969)J. Gen. Physiol.,33, 311.CrossRefGoogle Scholar
  18. Schachter, D., Dowdle, E. B. and Schenker, H. (1960)Am. J. Physiol.,198, 275.PubMedGoogle Scholar
  19. Schachter, D., Kimberg, D. V. and Schenker, H. (1961)Am. J. Physiol.,200, 1263.PubMedGoogle Scholar
  20. Schubert, J. and Lindenbaum, A. (1952)Am. Chem. Soc. J.,74, 3529.CrossRefGoogle Scholar
  21. Shane, A. M. (1963) inThe transfer of calcium and strontium across the biological membranes (ed. R. H. Wasserman) (New York, London: Academic Press) p. 359.Google Scholar
  22. Taylor, D. M. (1964) inAbstracts VIII of the 6th International Congress on Biochemistry, New York, Abstract No. 104.Google Scholar
  23. Taylor, D. M. (1967) inStrontium metabolism (eds J. M. A. Lenihan, J. F. Loutit and J. H. Martin) (London, New York: Academic Press) p. 175.Google Scholar
  24. Twardock, A. R. (1963) inThe transfer ofcalcium and strontium across the biological membranes (ed. R. H. Wasserman) (New York, London: Academic Press) p. 327.Google Scholar
  25. Twardock, A. R., Prinz, W. H. and Comar, C. L. (1960)Arch. Biochem. Biophys.,89, 309.PubMedCrossRefGoogle Scholar
  26. Walser, M. and Robinson, B. H. B. (1963) inThe transfer of calcium and strontium across the biological membranes (ed. R. H. Wasserman) (New York, London: Academic Press) p. 305.Google Scholar
  27. Wasserman, R. H. (1960)Proc. Soc. Exp. Biol. Med.,104, 92.PubMedGoogle Scholar
  28. Wasserman, R. H. (1964)Nature (London),201, 997.CrossRefGoogle Scholar
  29. Wasserman, R. H., Corradino, R. A. and Taylor, A. N. (1968)J. Biol. Chem.,243, 3978.PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • S. G. Kshirsagar
    • 1
  1. 1.Biochemistry DivisionBhabha Atomic Research CentreBombayIndia

Personalised recommendations