Abstract
The kinetics of lipase-catalyzed interesterification synthesis of L-ascorbyl lactate was studied. To determine the enzyme kinetic constants of the interesterification, a three-factor and five-level central composite design was used. The factors studied were ethyl lactate concentration, reaction temperature (T), and water content (w). Moreover, a statistical approach called the response surface method (RSM) was used to predict the kinetic constants. Finally, the relationships between the kinetic constants (V m and K m ) and the reaction parameters (T and w) were obtained.
To assess the accuracy of the RSM approach for determining V m and K m′ detailed validation experiments were carried out by the conventional approach at four different reaction parameters (35°C, 10μL; 45°C, 20μL; 55°C, 15 μL; 65°C, 18μL). The results indicated that the RSM approach gave reasonable results for the determination of Vm and Km in the range of tested parameters.
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Gao, J., Jiang, Y., Huang, Z. et al. Evaluation of kinetic parameters for enzymatic interesterification synthesis of L-ascorbyl lactate by response surface methodology. Appl Biochem Biotechnol 136, 153–164 (2007). https://doi.org/10.1007/BF02686020
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DOI: https://doi.org/10.1007/BF02686020