Skip to main content
SpringerLink
Log in

Kinetics of gas-phase hydrolysis of ethyl acetate catalyzed by immobilized lipase

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Reactions catalyzed by supported enzymes present important advantages when compared with those in aqueous media or organic solvents: separation of enzymes from substrate is easily accomplished, enzyme stability may be improved, and control of the reaction products is more accurate. We present the experimental results of the kinetic study of ethyl acetate hydrolysis in gaseous phase catalyzed by a commercial immobilized lipase (Lipozyme IM; Novo Nordisk). The hydrolysis reaction was studied as a function of ethyl ester and water partial pressure at a constant temperature of 318 K. The amount of biocatalyst used was varied between 100 and 300 mg, and the reaction was studied in a flow-through glass microreactor. Under the conditions used, water was an important parameter in the gas-phase reaction. Activation energy was 24.8 kJ/mol and the overall order of reaction was one. Finally a Bi-Bi reaction mechanism is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yang, F. X. and Russell, A. J. (1996), Biotechnol. Bioeng. 49, 709–716.

    Article  CAS  Google Scholar 

  2. Dordick, J. S. (1989), Enzyme Microb. Technol. 11, 194–211.

    Article  CAS  Google Scholar 

  3. Laane, C., Boeren, S., Vos, K., and Veeger, C. (1987), Biotechnol. Bioeng. 20, 81–87.

    Article  Google Scholar 

  4. Zaks, A. and Klibanov, A. (1885), Proc. Natl. Acad. Sci. USA 82, 3192–3196.

    Article  Google Scholar 

  5. Kazandjian, R. Z., Dordick, J. S., and Klibanov, A. (1986), Biotechnol. Bioeng. 28, 417–421.

    Article  CAS  Google Scholar 

  6. Nagamoto, H., Yasuda, T., and Inoue, H (1986), Biotechnol. Bioeng. 28, 1172–1177.

    Article  CAS  Google Scholar 

  7. Claon, P. A. and Akoh, C. C. (1994), J. Am. Oil Chem. Soc. 71, 575–578.

    Article  CAS  Google Scholar 

  8. Bárzana, E. (1996), Adv. Biochem. Eng. 53, 1–15.

    Google Scholar 

  9. Yagi, T., Tsuda, M., Mori, Y., and Inokuchi, H. (1969), J. Am. Chem. Soc. 91, 2801.

    Article  CAS  Google Scholar 

  10. Hou, C. T. (1984), Appl. Microbiol. Biotechnol. 19, 1–4.

    Article  CAS  Google Scholar 

  11. Hamstra, R. S., Murris, M. R., and Traper, J. (1987), Biotechnol. Bioeng. 29, 884–891.

    Article  CAS  Google Scholar 

  12. Uchiyama, H., Orugi, K., Yagi, O., and Kokufuta, E. (1992), Biotechnol. Lett. 14(7), 619–622.

    Article  CAS  Google Scholar 

  13. Maugard, T., Lamare, S., and Legoy, M. D. (2001), Biotechnol. Bioeng. 73(2), 164–168.

    Article  CAS  Google Scholar 

  14. Lamare, S. and Legoy, M. D. (1995), Biotechnol. Bioeng. 45, 387–397.

    Article  CAS  Google Scholar 

  15. Russell, A. J. and Yang, F. X. (1996), Chemtech 26, 24–27.

    CAS  Google Scholar 

  16. Paiva, A. L. and Malcata, F. X. (1997), J. Mol. Catal. B Enzymat. 3, 99–109.

    Article  CAS  Google Scholar 

  17. Yang, F. X. and Russell, A. J. (1996), Biotechnol. Bioeng. 49, 709–716.

    Article  CAS  Google Scholar 

  18. Lamare, S. and Legoy, M. D. (1993), Trends Biotechnol. 11, 413–418.

    Article  CAS  Google Scholar 

  19. Lamare, S., Robert, L., and Legoy, M. D. (1997), Biotechnol. Bioeng. 56, 1–8.

    Article  CAS  Google Scholar 

  20. Bárzana, E., Karel, M. and Klibanov, A. (1987), Appl. Biochem. Biotechnol. 15, 25–34.

    Google Scholar 

  21. Bárzana, E., Karel, M., and Klibanov, A. (1989), Biotechnol. Bioeng. 34, 1178–1185.

    Article  Google Scholar 

  22. Hwang, S. O., Trantolo, D. J., and Wise, D. L. (1993), Biotechnol. Bioeng. 42, 667–673.

    Article  CAS  Google Scholar 

  23. Hwang, S. O. and Park, Y. H. (1994), Biotechnol. Lett. 16, 379–384.

    CAS  Google Scholar 

  24. Hwang, S. O. and Park, Y. H. (1997), Bioprocess Eng. 17, 51–54.

    Article  CAS  Google Scholar 

  25. Parvaresh, F., Robert, H., Thomas, D., and Legoy, M. D. (1992), Biotechnol. Bioeng. 39, 467–473.

    Article  CAS  Google Scholar 

  26. Cameron, P. A., Davison, B. H., Frymier, P. D., and Barton, J. W. (2002), Biotechnol. Bioeng. 78, 251.

    Article  CAS  Google Scholar 

  27. Bousquet, M. P., Graber, M., Sousa, N., Lamare, S., and Legoy, M. D. (2001) Biochim. Biophys. Acta 1550, 90–99.

    Google Scholar 

  28. Graber, M., Bousquet-Dubouch, M. P., Lamare, S., and Legoy, M. D. (2003) Biochim. Biophys. Acta 1648, 24.

    CAS  Google Scholar 

  29. Graber, M., Bousquet, M. P., Sousa, N., Lamare, S., and Legoy, M. D. (2003) Biochim. Biophys. Acta 1645, 56–62.

    CAS  Google Scholar 

  30. Condoret, J. S., Vankan, S., and Joulia, X. (1997), Chem. Eng. Sci. 52(2), 213–220.

    Article  CAS  Google Scholar 

  31. Kim, C. and Rhee, S. (1992), Biotechnol. Lett. 14, 1059–1064.

    Article  CAS  Google Scholar 

  32. Boy, M., Dominik, A., and Voss, H. (1999), Process Biochem. 34, 535–547.

    Article  CAS  Google Scholar 

  33. Jaeger, K. E. and Reetz, M. T. (1998), Trends Biotechnol. 16, 396–403.

    Article  CAS  Google Scholar 

  34. Segel, I. H. (1975), Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, John Wiley & Sons, New York.

    Google Scholar 

  35. Segel, I. H. (1975), Biochemical Calculations, 2nd ed., John Wiley & Sons, New York.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnological Processes, State University of Campinas, PO Box 6066, 13083-970, Campinas, SP, Brazil

    Victor H. Perez

  2. Department of Biotechnological Processes, State University of Campinas, PO Box 6066, 13083-970, Campinas, SP, Brazil

    Everson A. Miranda

  3. Department of Chemical Processes, School of Chemical Engineering, State University of Campinas, PO Box 6066, 13083-970, Campinas, SP, Brazil

    Gustavo P. Valença

Authors
  1. Victor H. Perez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Everson A. Miranda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gustavo P. Valença
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Victor H. Perez.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perez, V.H., Miranda, E.A. & Valença, G.P. Kinetics of gas-phase hydrolysis of ethyl acetate catalyzed by immobilized lipase. Appl Biochem Biotechnol 136, 23–37 (2007). https://doi.org/10.1007/BF02685936

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02685936

Index Entries

Search

Navigation