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A protocol for non-radioactive DNA labelling and detection in the RFLP analysis of rice and tomato using single-copy probes

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Abstract

An improved protocol for non-radioactive labelling and detection of rice and tomato DNA is described. The procedure includes the use of polymerase chain reaction for the incorporation of digoxigenin-dUTP in the DNA molecule and the use of a chemiluminescent compound (AMPPD) for the signal detection.

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Abbreviations

AP:

alkaline phosphatase

dig:

digoxigen

PCR:

polymerase chain reaction

RFLP:

restriction fragment length polymorphism

References

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Author information

Authors and Affiliations

  1. Plant Breeding and Biometry Department, Cornell University, 252 Emerson Hall, 15853, Ithaca, NY, USA

    Olivier Panaud & Susan McCouch

  2. Plant Breeding Division, Genome Mapping Laboratory, International Rice Research Institute, P.O. Box 933, Manila, Philippines

    Gerard Magpantay

Authors
  1. Olivier Panaud
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  2. Gerard Magpantay
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  3. Susan McCouch
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Panaud, O., Magpantay, G. & McCouch, S. A protocol for non-radioactive DNA labelling and detection in the RFLP analysis of rice and tomato using single-copy probes. Plant Mol Biol Rep 11, 54–59 (1993). https://doi.org/10.1007/BF02670560

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  • DOI: https://doi.org/10.1007/BF02670560

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