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Elektronenmikroskopische Darstellung von strukturellen Einzelheiten in Nadelholz-Zellwänden anhand sehr dünner Ultramikrotomschnitte

Electron microscopic representation of structural details in softwood cell walls by very thin ultramicrotome sections

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Zusammenfassung

Eine Verbesserung der Einbettungs- und Kontrastierungsmethode für die Untersuchung von Holzgewebe im Transmissions-Elektronenmikroskop ermöglicht eine detaillierte Darstellung der Ligninverteilung in den verschiedenen Zellwandschichten. Untersuchungen an sehr dünnen Ultramikrotomschnitten von Fichtentracheiden zeigen, daß die stark lignifizierte „Mittelschicht” auch den äußeren Teil der Sekundärwand 1 umschließt. In der Sekundärwand 2 läßt sich an unbehandeltem Holz eine konzentrische Lamellierung sichtbar machen. In Druckholztracheiden konnte mit Hilfe der neuen Methode eine unregelmäßige lamellare Ligninverteilung in der S1 und der S2 gefunden werden.

Abstract

An improvement of the embedding and staining method for the study of wood tissue in the transmission electron microscope enables a detailed representation of the lignin distribution in the various cell wall layers. Studies of very thin sections of spruce tracheids show that the strongly lignified “compound middle lamella” includes also the outer part of the secondary wall 1. In the secondary wall 2 of untreated wood a concentric lamellation was made visible. An irregularly lamellar lignin distribution in the S1 and the S2 of compression wood tracheids could also be found by the new method.

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Literatur

  • Bachhuber, K.; Frösch, D. 1983: Melamine resins a new class of water soluble embedding media for electron microscopy. J. Microsc. 130:1–9.

    PubMed  CAS  Google Scholar 

  • Côté, W. A. 1977: Wood ultrastructure in relation to chemical composition. In: Loewus, F. A.; Runeckles, V. (ed) The structure, biosynthesis, and degradation of wood. Plenum Press, New York, London, pp. 1–44

    Google Scholar 

  • Côté, W.A.; Kutscha, N.P.; Simson, B.W.; Timell, T.E. 1968: Studies on compression wood. VI. Distribution of polysaccharides in the cell walls of tracheids of compression wood. Tappi 51:33–40

    Google Scholar 

  • Fergus, B.J.; Procter, A.R.; Scott, A.N.; Goring, D.A.I. 1969: The distribution of lignin in sprucewood as determined by ultraviolet microscopy. Wood Sci. Technol. 3:117–138

    Article  Google Scholar 

  • Harada, H. 1965: Ultrastructure and organization of gymnosperm cell walls. In: Côté, W.A. (ed.): Cellular ultrastructure of woody plants. Syracuse University Press, Syracuse, N.Y., pp. 215–233.

    Google Scholar 

  • Jayme, G.; Fengel, D. 1961: Beitrag zur Kenntnis des Feinbaus der Fichtentracheiden. II. Beobachtungen an Ultradünnschnitten von delignifiziertem Holz und Ligningerüsten. Holzfordschung 15:98–102

    Google Scholar 

  • Kerr, A.J.; Goring, A.A.I. 1975: The ultrastructural arrangement of the wood cell wall. Cell. Chem. Technol. 9:563–573

    Google Scholar 

  • Liese, W. 1965: The Warty Layer In: Côté, W.A. (ed.) Cellular ultrastructure of woody plants. Syracuse University Press, Syracuse, N.Y., pp. 251–269

    Google Scholar 

  • Maurer, A.; Fengel, D. 1990a: A new process for improving the quality and lignin staining of ultrathin sections from wood tissue. Holzforschung 44:455–460

    Article  Google Scholar 

  • Maurer, A.; Fengel, D. 1990b: On the solubility of lignin in various cell wall layers. 1st Europ. workshop on lignoscellulosics and pulp, Proceed. Hamburg (im Druck)

  • Ruel, K.; Barnoud, F.; Goring, D.A.I. 1978: Lamellation in the S2 layer of softwood tracheids as demonstrated by scanning transmission electron microscopy. Wood Sci. Technol. 12:287–291

    Article  Google Scholar 

  • Sachs, I.B.; Clark, I.T.; Pew, J.C. 1963: Investigation of lignin distribution in the cell wall of certain woods. J. Polymer Sci., Pt. C, No. 2:203–212

    Google Scholar 

  • Saka, S.; Thomas, R.J.; Gratzl, J.S. 1978: Lignin distribution. Determination by energy dispersive analysis of X-rays. Tappi 61(1):73–76

    CAS  Google Scholar 

  • Scott, J.A.N.; Goring, D.A.I. 1970: Lignin concentration in the S, layer of softwoods. Cell. Chem. Technol. 4:83–93

    CAS  Google Scholar 

  • Timell, T.E. 1978: Ultrastructure of compression wood in Ginkgo biloba. Wood Sci. Technol. 12:89–103

    Google Scholar 

  • Westermark, U.; Lidbrandt, O.; Eriksson, I. 1988: Lignin distribution in spruce (Picea abies) determined by mercurization with SEM-EDXA technique. Wood. Sci. Technol. 22:243–250

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Instut für Holzforschung der Universität München, Winzererstraße 45, W-8000, München 40

    A. Maurer & D. Fengel

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  1. A. Maurer
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  2. D. Fengel
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Herrn Prof. Dr. Dr. h. c. mult. Walter Liese anläßlich seines 65. Geburtstages gewidmet

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Maurer, A., Fengel, D. Elektronenmikroskopische Darstellung von strukturellen Einzelheiten in Nadelholz-Zellwänden anhand sehr dünner Ultramikrotomschnitte. Holz als Roh-und Werkstoff 49, 53–56 (1991). https://doi.org/10.1007/BF02662800

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