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Chromatographische Untersuchungen zur quantitativen Bestimmung der Holzzucker

Comparative chromatographic investigations for quantitative determination of wood sugars

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Zusammenfassung

Drei chromatographische Methoden, nämlich die Boratkomplex-Anionenaustauschchromatographie, die Anionenaustausch-chromatographie in NaOH-Medium und die Hochleistungsdünnschichtchromatographie werden am Beispiel saurer Hydrolysate von Buchenholz, Fichtenholz und Chinaschilf auf ihre Eignung zur Quantifizierung der fünf Holzzucker Galactose, Glucose, Mannose, Arabinose und Xylose überprüft.

Abstract

Three chromatographic methods for determination of wood sugars are compared. These are borate complex anionexchange chromatography, anionexchange chromatography in NaOH medium and high performance thin layer chromatography. Acid hydrolysates of beech, spruce and Miscanthus were taken as an example for quantitative separation of galactose, glucose, mannose, arabinose and xylose.

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Literatur

  • Batisse, C.;Daurade, M. H.;Bounias, M.. 1992: TLC separation and spectrodensitometric quantification of cell wall neutral sugars. J. Planar. Chromatogr. 5: 131–133

    CAS  Google Scholar 

  • Baker, J. O.;Himmel, M. E.. 1986: Separation of sugar anomers by aqueous chromatography on calcium- and lead-form ion-exchange columns. Application to anomeric analysis of enzyme reaction products. J. Chromatogr. 357: 161–181.

    Article  CAS  Google Scholar 

  • Bauer, H.;Voelter, W.. 1976: Ionenaustausch-Chromatographie von Kohlen-hydraten. Chromatographia 9: 433–439.

    Article  CAS  Google Scholar 

  • Binder, H.. 1980: Separation of monosaccharides by high-performance liquid chromatography: Comparison of ultraviolett and refractive index detection. J. Chromatogr. 189: 414–420

    Article  CAS  Google Scholar 

  • Bochter, R.. 1984: Bestimung cellulosisch und nichtcellulosisch gebundener Neutralzucker in Bodenhydrolysaten mit Hilfe von Hochleistungsdünnschichtchromatographie. Z. Pflanzenernähr. Bodenk. 147: 203–209

    Article  CAS  Google Scholar 

  • Bonn, G.. 1985: High-performance liquid chromatographic elution behavior of oligosaccharides, monosaccharides and sugar-degradation products on series-connected ion-exchange resin columns using water as mobile phase. J. Chromatogr. 322: 411–424.

    Article  CAS  Google Scholar 

  • Del Nozal, M. J.;Bernal, J. L.;Gomes, F. J.;Antolin, A.;Toribio, L.. 1992: Post-column derivatization of carbohydrates with ethanolamine-boric acid prior to their detection by high-performance liquid chromatography. J. Chromatogr. 607: 31–198

    Article  Google Scholar 

  • Doerffel, K. 1987: Statistik in der analytischen Chemie. Kap. 4. Zufallsfehler von Analysenverfahren. Weinheim: VCH

    Google Scholar 

  • Edwards, W. T.;Pohl, C. A.;Rubin, R.. 1987: Determination of carbohydrates using pulsed amperometric detection combined with anion exchange separations. Tappi 70, 6: 138–140.

    CAS  Google Scholar 

  • Fengel, D.;Wegener, G. 1979: Hydrolysis of polysaccharides with trifluoroacetic acid and its application to rapid wood and pulp analysis. In Hydrolysis of cellulose: Mechanisms of enzymatic and acid hydrolysis (Adv. Chem. Ser. 181). Washington: ACS, S. 145–158

    Google Scholar 

  • Fengel, D.;Przyklenk, M.. 1993: Pretreatment of cellulose-containing samples for total hydrolysis with trifluoroacetic acid. Holz Roh-Werkst. 51: 294

    CAS  Google Scholar 

  • Fengel, D.;Wegener, G.. 1984: Wood-Chemistry, Ultrastructure, Reactions. Berlin, New York: Walter de Gruyter

    Google Scholar 

  • Ghebregzabher, M.;Rufini, S.;Monadi, B.;Lato, M.. 1976: Thin-layer chromatography of carbohydrates. J. Chromatogr. 127: 133–162

    Article  PubMed  CAS  Google Scholar 

  • Jork H.;Fischer, W.;Wimmer, H.. 1989. Dünnschichtchromatographie Reagentien und Nachweismethoden, Bd. 1a, Weinheim, VCH

    Google Scholar 

  • Kaar, W. E.;Cool, L. G.;Merriman, M. M.;Brink, D. L.. 1991: The complete analysis of wood polysaccharides using HPLC. J. Wood Chem. Technol. 11: 447–463

    CAS  Google Scholar 

  • Körner, H.-U.;Gottschalk, D.;Wiegel, J.;Puls, J.. 1984: The degradation pattern of oligomers and polymers from lignocelluloses. Anal. Chem. Acta 63: 55–66

    Article  Google Scholar 

  • Laver, M. L.;Wilson, K. P.. 1993: Determination of carbohydrates in wood pulp products, Tappi 76: 155–159

    CAS  Google Scholar 

  • McGinnis, G. D.;Biermann, C. J.. 1989: Analysis of monosaccharides as per-o-acetylated aldononitrile (PAAN) derivatives by gas-liquid-chromatography (GLC). In: Analysis of carbohydrates by GLC and MS. Hrsg: Biermann, C. J.; McGinnis, G.D., Boca Raton: CRC Press, S. 119–125.

    Google Scholar 

  • McGinnis, G. D.;Laver, M. L.;Biermann C. J.. 1989: High-performance liquid chromatography (HPLC) of carbohydrates. In: Analysis of carbohydrates by GLC and MS. Hrsg: Biermann, C. J.; McGinnis, G. D., Boca Raton: CRC Press, S. 19–26

    Google Scholar 

  • Nieschlag, H. J.;Nelson, G. H.;Wolf, I. A.;Perdue Jr., R. E. 1960: A search for new fiber crops. Tappi 43(3): 193–201.

    CAS  Google Scholar 

  • Paice, M. G.;Jurasek, L.;Desrocher, M. 1982: Simplified analysis of wood sugars. Tappi 65, 7: 103–106

    Google Scholar 

  • Patzsch, K.;Netz, S.;Funk, W.. 1988: Quantitative HPTLC of sugars: Part 1: Separation and derivatisation. J. Planar. Chromatogr. 1: 39–45.

    CAS  Google Scholar 

  • Pettersen, R. C. 1984: The chemical composition of wood. In: The chemistry of solid wood, Hrsg: Rowell, R, Washington: ACS, S. 57–126

    Google Scholar 

  • Pettersen, R. C.;Schwandt, V. H. 1991: Wood sugar analysis by anion chromatography. J. Wood Chem. Technol. 11: 495–501

    CAS  Google Scholar 

  • Pruden, B. B.;Pineault, G.. 1975: A thin-layer chromatographic method for the quantitative determination of D-mannose, D-glucose and D-galactose in aqueous solution. J. Chromatogr. 115: 477–483

    Article  CAS  Google Scholar 

  • Puls, J. 1993: Substrate analysis of forest and agriculture wastes. In: Bioconversion of forest and agricultural plant residues. Hrsg: Saddler, J. N. Wallingford: CAB International, 13–32

    Google Scholar 

  • Saeman, J. F.;Moore, W. E.;Mitchell, R. L.;Millett, M. A. 1954: Technique for the determination of pulp constituents by quantitative paper chromatography. Tappi 37: 336–343

    CAS  Google Scholar 

  • Sinner, M.;Puls, J.. 1978: Non-corrosive dye reagent for detection of reducing sugars in borate complex ion-exchange chromatography. J. Chromatogr. 156: 197–204

    Article  CAS  Google Scholar 

  • Sinner, M.;Simatupang, M. H.;Dietrichs, H. H.. 1975: Automated quantitative analysis of wood carbohydrates by borate complex exchange chromatography. Wood Sci. Technol. 9: 307–322

    Article  CAS  Google Scholar 

  • Simatupang, M. H.;Dietrichs, H. H.. 1978: Neocuproin als Nachweisreagenz reduzierender Zucker bei der lonenaustausch-Chromatographie. Chromatographia 11: 89–95

    Article  CAS  Google Scholar 

  • Simson, B. W.;Timell, T. E.. 1967: A method for determination of the carbohydrate composition of microgram quantities of plant tissues. Tappi 50, 10: 473–477

    CAS  Google Scholar 

  • Timell, T. E.. 1957: Carbohydrate composition of ten North American species of wood. Tappi 40, 7: 568–572

    CAS  Google Scholar 

  • Timell, T. E. 1967: Recent progress in the chemistry of wood hemicelluloses. Wood Science Technol. 1: 45–70

    Article  CAS  Google Scholar 

  • Van Riel, J. A. M.;Olieman, C. 1986: HPLC of sugars on a mixed cation-exchange resin column. J. Chromatogr. 362: 235–242

    Article  Google Scholar 

  • Washüttl, J.;Riederer, P.;Bancher, E.;Wurst, F.;Steiner, K.. 1974: Dünnschicht-und Gas chromatographie natürlich vorkommender Gemische von Zuckern und Zuckeralkoholen. Z. Lebensm. Unters.-Forsch. 155: 77–80

    Article  Google Scholar 

  • Wentz, F. E.;Marcy, A. D.;Gray, M. J.. 1982: Analysis of wood sugars in pulp and paper industry samples by HPLC. J. Chromatogr. Sci. 20: 349–352

    CAS  Google Scholar 

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Herrn Prof. Dr. D. Fengel anläßlich seines Ausscheidens aus dem Dienst gewidmet

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Uremovic, A., Dokk Glawischnig, T., Schuseil, J. et al. Chromatographische Untersuchungen zur quantitativen Bestimmung der Holzzucker. Holz als Roh-und Werkstoff 52, 347–354 (1994). https://doi.org/10.1007/BF02615383

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