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The influence of a fish oil-enriched diet on the phospholipid fatty acid turnover in the rabbit red cell membranein vivo

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Lipids

Abstract

The influence of the polyunsaturated fatty acid (PUFA) composition of the diet on the rate of fatty acid turnover of individual phospholipids in the erythrocyte membranein vivo was studied. Following modification of the fatty acid composition of the membrane phospholipids by the use of a fish oil or a linoleic acid enriched diet, phospholipids—labelled in the unsaturated fatty acid at the 2-position of the glycerol moiety—were introduced into the membrane of freshly isolated rabbit erythrocytes. Thereafter, the labelled erythrocytes were reinjected into the bloodstream of the animal. It appears that, with the exception of 1-palmitoyl,2-linoleoyl phosphatidylcholine, all other phosphatidylcholines disappear faster from the erythrocytes of fish oil-fed rabbits than from the red cells of linoleic acid-fed rabbits. Another parameter, which possibly influences the turnover rates of PUFA containing phospholipids, can be peroxidation. An attempt was made to measure peroxidative damage of lipidsin vivo by the introduction of 1-palmitoyl,2-cis-parinaroyl phosphatidylcholine (PnPC)—a probe to measure oxidative stress—into the membrane of freshly isolated erythrocytes, in the same way as is described for the radioactive phospholipids. The data demonstrate that the fluorescent signal from the PnPC decreases at a fast rate which is independent of the dietary conditions.

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Abbreviations

ACD:

acid-citrate-dextrose

BHT:

butylhydroxy toluene

nsL-TP:

nonspecific lipid transfer protein

PC:

phosphatidylcholine

16:0,18:1PC:

1-palmitoyl,2-oleoyl-sn-glycero-3PC

16:0,18:2PC:

1-palmitoyl,2-linoleoyl-sn-glycero-3PC

18:0,20:4PC:

1-stearoyl,2-arachidonoyl-sn-glycero-3PC

PC-TP:

phosphatidylcholine specific transfer protein

PE:

phosphatidylethanolamine

16:0,20:4PE:

1-palmitoyl 1,2-arachidonoyl-sn-glycero-3PE

PnA:

parinaric acid

PnPC:

1-palmitoyl,2-cis-parinaroyl PC

PUFA:

polyunsaturated fatty acid(s)

α-TE:

R,R,R,-α-tocopherol

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Authors and Affiliations

  1. Department of Biochemistry of Lipids, Centre for Biomembranes and Lipid Enzymology, Institute for Biomembranes, Utrecht University, P.O. Box 80054, 3508 TB, Utrecht, The Netherlands

    M. A. P. van den Boom, M. Groot Wassink, B. Roelofsen & J. A. F. Op den Kamp

  2. Department of Biosciences, Nutrition and Safety, Unilever Research Laboratory, Vlaardingen, The Netherlands

    N. J. de Fouw

Authors
  1. M. A. P. van den Boom
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  2. M. Groot Wassink
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  3. B. Roelofsen
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  4. N. J. de Fouw
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  5. J. A. F. Op den Kamp
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van den Boom, M.A.P., Groot Wassink, M., Roelofsen, B. et al. The influence of a fish oil-enriched diet on the phospholipid fatty acid turnover in the rabbit red cell membranein vivo . Lipids 31, 285–293 (1996). https://doi.org/10.1007/BF02529875

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  • DOI: https://doi.org/10.1007/BF02529875

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