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Selection of protoclones for high yields of indole alkaloids from suspension cultures ofCatharanthus roseus and the qualitative analysis of the compounds by LC-MS

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Biotechnology Techniques

Summary

To produce economically important indole alkaloids by cell culture, we have selected protoclones ofCatharanthus roseus for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. Protoclone VPC-10 produced catharanthine at 5.9 μg/g fresh wt of cells after 10 days of culture, although the original cell line did not produce it at a level detectable by HPLC. Under the same conditions, protoclone VPC-15 produced ajmalicine at 133.6 μg/g, which was about 3 times the productivity of the original cell line. In addition, the indole alkaloids were qualitatively confirmed by LC-MS.

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Authors and Affiliations

  1. Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taedok Science Town, P.O. Box 17, 305-606, Taejon, Korea

    K. H. Jung, S. S. Kwak, S. W. Kim & J. R. Liu

  2. Biochemical Engineering Major, Seoul National University, 151-742, Seoul, Korea

    K. H. Jung & C. Y. Choi

  3. Korea Standard Research Institute, Taedok Science Town, P.O. Box 3, 305-606, Taejon, Korea

    G. S. Heo

Authors
  1. K. H. Jung
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  2. S. S. Kwak
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  3. S. W. Kim
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  4. C. Y. Choi
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  5. G. S. Heo
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  6. J. R. Liu
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Jung, K.H., Kwak, S.S., Kim, S.W. et al. Selection of protoclones for high yields of indole alkaloids from suspension cultures ofCatharanthus roseus and the qualitative analysis of the compounds by LC-MS. Biotechnol Tech 6, 305–308 (1992). https://doi.org/10.1007/BF02439316

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  • DOI: https://doi.org/10.1007/BF02439316

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