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The [53–63] amino acid portion of the parathyroid hormone molecule is essential for the stimulatory effect of carboxyl-terminal PTH fragments on alkaline phosphatase activity in dexamethasone-treated rat osteoblastic osteosarcoma cells, ROS 17/2.8

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Abstract

The effect of a series of truncated carboxyl-terminal parathyroid hormone (PTH) fragments on alkaline phosphatase (ALP) activity was further examined in dexamethasone-treated rat osteoblastic osteosarcoma cells, ROS 17/2.8. As we previously reported, dexamethasone-induced ALP activity was inhibited not only by hPTH(1-84) and aminoterminal PTH fragment hPTH(1-34), but also by carboxyl-terminal PTH fragment hPTH(69-84). The longer carboxyl-terminal PTH fragment hPTH(53-84) stimulated ALP activity, and the shorter carboxyl-terminal PTH fragment hPTH(71-84) did not affect ALP activity.

The longest newly synthesized carboxyl-terminal PTH fragment hPTH(35-84), which is complementary to amino-terminal PTH fragment hPTH(1-34), stimulated ALP activity as potently as hPTH(53-84), but not more potently than hPTH(53-84). Another newly synthesized carboxyl-terminal PTH fragment hPTH(64-84), which has an intermediate peptide length between hPTH (53-84) and hPTH(69-84), inhibited ALP activity as potently as hPTH(69-84).

These results suggest that the 35-52 amino acid portion of the PTH molecule might not be crucial for the stimulatory effect of carboxyl-terminal PTH fragments on ALP activity, and that the 53–63 portion, but not the 64–68 portion, of the PTH molecule might be essential for the stimulatory effect of carboxyl-terminal PTH fragments on ALP activity. Furthermore, the importance of the 69th and 70th amino acid of the PTH molecule for the inhibitory effect of carboxyl-terminal PTH fragments on ALP activity was confirmed.

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Authors and Affiliations

  1. Third Division, Department of Medicine, Kobe University, Kobe, Japan

    Hisamitsu Baba M.D., Chizu Nakamoto & Kazuo Chihara

  2. Peptide Institute Inc. Protein Research Foundation, Osaka, Japan

    Kiichiro Nakajima, Terutoshi Kimura & Shumpei Sakakibara

  3. Katsuragi Hospital Calcium Research Institute, Osaka, Japan

    Takuo Fujita

  4. Chugai Pharmaceutical Co. Ltd., Shizuoka & Tokyo, Japan

    Kenji Kumaki & Takashi Mori

  5. Department of Biosignal Pathophysiology & Medical Center for Students' Health Service, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657, Kobe, Japan

    Hisamitsu Baba M.D. & Isamu Nagai

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  1. Hisamitsu Baba M.D.
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  2. Chizu Nakamoto
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  3. Kiichiro Nakajima
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  4. Terutoshi Kimura
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  5. Shumpei Sakakibara
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  7. Kenji Kumaki
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  9. Kazuo Chihara
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Baba, H., Nakamoto, C., Nakajima, K. et al. The [53–63] amino acid portion of the parathyroid hormone molecule is essential for the stimulatory effect of carboxyl-terminal PTH fragments on alkaline phosphatase activity in dexamethasone-treated rat osteoblastic osteosarcoma cells, ROS 17/2.8. J Bone Miner Metab 12 (Suppl 1), S139–S143 (1994). https://doi.org/10.1007/BF02375692

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  • DOI: https://doi.org/10.1007/BF02375692

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