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Isolationin vitro ofStrongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies,Delia radicum [Dipt.: Anthomyiidae]

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Abstract

The entomogenous fungusStrongwellsea castrans was isolatedin vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media.

Using the API-ZYM system, tests on extracts on hyphae ofS. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles betweenS. castrans and fungi of the related genusErynia.

Résumé

Le champignon entomopathogèneStrongwellsea castrans a été isolé pour la première foisin vitro en incubant des conidies projetées de cadavres de mouches du chou (Delia radicum) dans un milieu liquide simple et semi-défini contenant du dextrose, de l'extrait de levure et de l'hydrolysat de lactalbumine tamponné à pH 7. Le champignon s'y est développé sous forme d'hyphes longues et unituniquées. Après transfert sur milieu solide, des corps hyphaux multinucléés sont apparus et ont développé une paroi plus épaisse et laminée. On n'observera aucune conidie ni spore de résistance, que ce soit en milieu liquide ou sur milieu solide, et le champignon ne survécut aux repiquages qu'en milieu liquide.

En utilisant le système API-ZYM, des tests sur des extraits d'hyphes deS. castrans se sont avérés positifs pour 11 enzymes mais aucune différence nette n'a été observée en ce qui concerne les profils d'enzymes entreS. castrans et des champignons du genreErynia.

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Authors and Affiliations

  1. Department of Zoology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK 1870, Frb. C, Denmark

    J. Eilenberg & J. Bresciani

  2. Entomology and Nematology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., United Kingdom

    N. Wilding

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  1. J. Eilenberg
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  2. N. Wilding
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  3. J. Bresciani
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Eilenberg, J., Wilding, N. & Bresciani, J. Isolationin vitro ofStrongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies,Delia radicum [Dipt.: Anthomyiidae] . Entomophaga 37, 65–77 (1992). https://doi.org/10.1007/BF02372975

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