Abstract
The contamination caused by rubber stoppers of collection tubes preceding the determination of zinc in plasma and serum was investigated. The use of evacuated blood collection tubes (EBC-tubes), normally used for the collection of serum samples, could give rise to an average artificial increase of normal serum zinc concentrations of about 250%. Heparine containingebc-tubes, used for the collection of plasma, and plainebc-tubes of an experimental production appeared to be suitable for correct zinc determinations. There was no difference between the serum and plasma zinc levels of fifty volunteers.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Riordan JF, Vallee BL. Structure and function of zinc metalloenzymes. In: Prasad AS, Oberleas D, eds. Trace elements in human health and disease, Vol. 1: Zinc and copper. New York: Academic Press, 1976:227–56.
Prasad AS, Miale A Jr, Farid Z, Sandstead HH, Schulert AR, Darby WJ. Biochemical studies on dwarfism, hypogonadism and anemia. Arch Intern Med 1963;111:407–28.
Sommer AL, Lipman CB. Evidence on the indispensable nature of zinc and boron for higher green plants. Plant Phys 1926;1:231–49.
Underwood EJ. Trace elements in human and animal nutrition. 4th ed. New York: Academic Press, 1977.
Hambidge KM, Hambidge C, Jacobs M, Baum JD. Low levels of zinc in hair, anorexia, poor growth and hypgeusia in children. Pediatr Res 1972;6:868–74.
Halsted JA, Smith JC. Plasma zinc in health and disease. Lancet 1970;1:322–4.
Weismann K, Roed-Peterson J, Hjorth N. Chronic zinc deficiency syndrome in a beer drinker with a Billroth II resection. Int J Dermatol 1976;15:757–61.
Elmes M, Golden MK, Love AHG. Unresponsive coeliac disease. Q J Med 1976;45:696–7.
Mikac-Dević D. Methodology of zinc determinations and the role of zinc in biochemical processes. Adv Clin Chem 1970;13:271–333.
Foley B, Johnson SA, Hackley B, Smith JC Jr, Halsted JA. Zinc content of human platelets. Proc Soc Exp Biol Med 1968;128:265–9.
Kiilerich S, Christensen MS, Naestoft J, Christiansen C. Serum and plasma zinc concentrations with special reference to standardized sampling procedure and protein status. Clin Chim Acta 1981;114:117–21.
Reimold JG, Besch DJ. Detection and elimination of contaminations interfering with the determination of zinc in plasma. Clin Chem 1978;24:675–80.
Williams DM. Trace metal determinations in blood obtained in evacuated collection tubes. Clin Chim Acta 1979;99:23–9.
Smith JC Jr, Butrimovitz GP, Purdy WC. Direct measurement of zinc in plasma by atomic absorption spectroscopy. Clin Chem 1979;25:1487–91.
Deming WE. Statistical adjustment of data. New York: John Wiley & Sons, Ltd., 1943:184.
Kiilerich S, Christensen MS, Naestoft J, Christiansen C. Determination of zinc in serum and urine by atomic absorption spectrophotometry; relationship between serum levels of zinc and proteins in 104 normal subjects. Clin Chim Acta 1980;105:231–9.
Casey CE, Hambidge KM. Epidemiological aspects of human zinc deficiency. In: Nriagu JO, ed. Zinc in the environment, part II: Health effects. New York: John Wiley & Sons, Ltd., 1980.
Whitehouse RC, Prasad AS, Rabbani PI, Cossack ZT. Zinc in plasma, neutrophils, lymphocytes, and erythrocytes as determined by flameless atomic absorption spectrophotometry. Clin Chem 1982;28:475–80.
Kasarskis EJ, Schuna A. Serum alkaline phosphate after treatment of zinc deficiency in humans. Am J Clin Nutr 1980;33:2609–12.
Author information
Authors and Affiliations
Additional information
In honour of Professor Faber on the occasion of his 25-years' professorate.
Rights and permissions
About this article
Cite this article
Keyzer, J.J., Oosting, E., Wolthers, B.G. et al. Zinc in plasma and serum: influence of contamination due to the collection tubes. Pharmaceutisch Weekblad Scientific Edition 5, 248–251 (1983). https://doi.org/10.1007/BF02332953
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02332953