Pharmaceutisch Weekblad

, Volume 5, Issue 5, pp 248–251 | Cite as

Zinc in plasma and serum: influence of contamination due to the collection tubes

  • J. J. Keyzer
  • E. Oosting
  • B. G. Wolthers
  • F. A. J. Muskiet
  • F. R. Hindriks
  • W. van der Slik
Original Articles


The contamination caused by rubber stoppers of collection tubes preceding the determination of zinc in plasma and serum was investigated. The use of evacuated blood collection tubes (EBC-tubes), normally used for the collection of serum samples, could give rise to an average artificial increase of normal serum zinc concentrations of about 250%. Heparine containingebc-tubes, used for the collection of plasma, and plainebc-tubes of an experimental production appeared to be suitable for correct zinc determinations. There was no difference between the serum and plasma zinc levels of fifty volunteers.


Zinc Serum Sample Stopper Normal Serum Blood Collection 
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  1. 1.
    Riordan JF, Vallee BL. Structure and function of zinc metalloenzymes. In: Prasad AS, Oberleas D, eds. Trace elements in human health and disease, Vol. 1: Zinc and copper. New York: Academic Press, 1976:227–56.Google Scholar
  2. 2.
    Prasad AS, Miale A Jr, Farid Z, Sandstead HH, Schulert AR, Darby WJ. Biochemical studies on dwarfism, hypogonadism and anemia. Arch Intern Med 1963;111:407–28.PubMedGoogle Scholar
  3. 3.
    Sommer AL, Lipman CB. Evidence on the indispensable nature of zinc and boron for higher green plants. Plant Phys 1926;1:231–49.Google Scholar
  4. 4.
    Underwood EJ. Trace elements in human and animal nutrition. 4th ed. New York: Academic Press, 1977.Google Scholar
  5. 5.
    Hambidge KM, Hambidge C, Jacobs M, Baum JD. Low levels of zinc in hair, anorexia, poor growth and hypgeusia in children. Pediatr Res 1972;6:868–74.PubMedGoogle Scholar
  6. 6.
    Halsted JA, Smith JC. Plasma zinc in health and disease. Lancet 1970;1:322–4.PubMedGoogle Scholar
  7. 7.
    Weismann K, Roed-Peterson J, Hjorth N. Chronic zinc deficiency syndrome in a beer drinker with a Billroth II resection. Int J Dermatol 1976;15:757–61.PubMedGoogle Scholar
  8. 8.
    Elmes M, Golden MK, Love AHG. Unresponsive coeliac disease. Q J Med 1976;45:696–7.Google Scholar
  9. 9.
    Mikac-Dević D. Methodology of zinc determinations and the role of zinc in biochemical processes. Adv Clin Chem 1970;13:271–333.PubMedGoogle Scholar
  10. 10.
    Foley B, Johnson SA, Hackley B, Smith JC Jr, Halsted JA. Zinc content of human platelets. Proc Soc Exp Biol Med 1968;128:265–9.PubMedGoogle Scholar
  11. 11.
    Kiilerich S, Christensen MS, Naestoft J, Christiansen C. Serum and plasma zinc concentrations with special reference to standardized sampling procedure and protein status. Clin Chim Acta 1981;114:117–21.CrossRefPubMedGoogle Scholar
  12. 12.
    Reimold JG, Besch DJ. Detection and elimination of contaminations interfering with the determination of zinc in plasma. Clin Chem 1978;24:675–80.PubMedGoogle Scholar
  13. 13.
    Williams DM. Trace metal determinations in blood obtained in evacuated collection tubes. Clin Chim Acta 1979;99:23–9.CrossRefPubMedGoogle Scholar
  14. 14.
    Smith JC Jr, Butrimovitz GP, Purdy WC. Direct measurement of zinc in plasma by atomic absorption spectroscopy. Clin Chem 1979;25:1487–91.PubMedGoogle Scholar
  15. 15.
    Deming WE. Statistical adjustment of data. New York: John Wiley & Sons, Ltd., 1943:184.Google Scholar
  16. 16.
    Kiilerich S, Christensen MS, Naestoft J, Christiansen C. Determination of zinc in serum and urine by atomic absorption spectrophotometry; relationship between serum levels of zinc and proteins in 104 normal subjects. Clin Chim Acta 1980;105:231–9.CrossRefPubMedGoogle Scholar
  17. 17.
    Casey CE, Hambidge KM. Epidemiological aspects of human zinc deficiency. In: Nriagu JO, ed. Zinc in the environment, part II: Health effects. New York: John Wiley & Sons, Ltd., 1980.Google Scholar
  18. 18.
    Whitehouse RC, Prasad AS, Rabbani PI, Cossack ZT. Zinc in plasma, neutrophils, lymphocytes, and erythrocytes as determined by flameless atomic absorption spectrophotometry. Clin Chem 1982;28:475–80.PubMedGoogle Scholar
  19. 19.
    Kasarskis EJ, Schuna A. Serum alkaline phosphate after treatment of zinc deficiency in humans. Am J Clin Nutr 1980;33:2609–12.PubMedGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1983

Authors and Affiliations

  • J. J. Keyzer
    • 1
  • E. Oosting
    • 1
  • B. G. Wolthers
    • 1
  • F. A. J. Muskiet
    • 1
  • F. R. Hindriks
    • 1
  • W. van der Slik
    • 1
  1. 1.Central Laboratory for Clinical ChemistryUniversity HospitalRB GroningenThe Netherlands

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