Summary
A rapid and simple method has been developed for the group fractionation of the major unsulphated and mono-sulphated bile acids in human body fluids. After extraction with Bond Elut C18 cartridges, the bile acids are separated into the unconjugated, glycine-, taurine- and sulphate-conjugated forms on pre-packed Bond Elut SAX columns by increasing the ionic strength of the methanol-acetate buffer eluent. The procedure was found to be accurate and reproducible and to give complete resolution between the different groups. The levels of 3-sulphate bile acids in human serum and urine from patients with liver disease were determined by high-performance liquid chromatography, after group separation and solvolysis of the sulphate fraction.
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Scalia, S., Pazzi, P. Group separation of non-sulphated and 3-sulphated bile acids by disposable anion-exchange cartridges. Chromatographia 30, 377–381 (1990). https://doi.org/10.1007/BF02328501
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DOI: https://doi.org/10.1007/BF02328501