Summary
HPTLC densitometry and HPLC are considered for the simultaneous determination of the degradation products of piroxicam (2-aminopyridine, DP-I and DP-II). The substances were separated on silica gel with fluorescence indicator in ethylacetate — toluene — diethylamine (10∶10∶5) and toluene — absolute ethanol — glacial acetic acid (8∶1.2∶0.5) systems. The measuring absorbance (detection of reflectance) of the separated substances was carried out “in situ” at 296 nm using 4-level calibration (external standard, nonlinear regresson function) in the concentration range 600–1200 ng 2-aminopyridine/spot and 300–600 ng DP-I and DP-II/spot. The HPLC method was carried out using RP-8 stationary phase and methanol + phosphate-citrate buffer, pH 3 mobile phase with addition of sodium pentanesulfonate (40+60, v/v). 2-aminopyridine wass detected at 300 nm, DP-I at 280 nm and DP-II at 248 nm. The concentration range for 2-aminopyridine is 2–40 μg/ml, for DP-I and DP-II 2–20 μg/ml (for an injection volume of 10 μl). The results were evaluated by linear regression analysis.
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References
H. Zinnes, J. C. Sircar, N. Lundo, A. C. Fabien, J. Shavel, Jr., C. F. Kasulanis, J. D. Genzer, C. Lutonski, D. Di Pasquale, J. Med. Chem.,25, 12 (1982).
R. N. Brogden, R. C. Heel, T. M. Speight, G. S. Avery, Drugs,28, 292 (1984).
T. M. Twomey, S. R. Bartolucci, D. C. Hobbs, J. Chromatogr.,183, 104 (1980).
P. Schiantarelli, D. Acerbi, G. Bovis, Arzneim.-Forsch./Drug Res.,31, 92 (1981).
J. B. Fourtillan, D. Dubourg, Thérapie,38, 163 (1983).
H. Laufen, K. D. Riedel, K. Räder, A. Schwedass, Fresenius Z. Anal. Chem.,318, 257 (1984).
J. S. Dixon, J. R. Lowe, D. B. Galloway, J. Chromatogr.,310, 455 (1984).
C. J. Richardson, S. G. Ross, K. L. Blocka, R. K. Verbeeck, J. Chromatogr.,382, 382 (1986).
J. Macek, J. Vácha, J. Chromatogr.,420, 445 (1987).
F. DDouglas Boudinot, S. S. Ibrahim, J. Chromatogr.,430, 424 (1988).
J. Vácha, J. Macek, H. Tománková, J. Jĩrincová, J. Elis, Českosiov. Farm.38, No. 5 (1989).
K. D. Riedel, H. Laufen, J. Chromatogr.,276, 243 (1983).
D. C. Hobbs, T. M. Twomey, J. Clin. Pharmacol.,19, 270 (1979).
F. Kozjek, L. Golič, P. Zupet, E. Palka, P. Vodopivec, M. Japelj, Acta Pharm. Jugosl.,35, 275 (1985).
M. ÄÄrynen, J. Palho, Arzneim.-Forsch./Drug Res.,36, 744 (1986).
J. A. Richards, D. A. Cole, R. J. Hickey, L. S.-W. Sokol, J. Chromatogr. Sci.,25, 292 (1987).
USP XXI, Second Supplement, p. 1880 (1985).
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Tománková, H., Šabartová, J. Determination of potential degradation products of piroxicam by HPTLC densiometry and HPLC. Chromatographia 28, 197–202 (1989). https://doi.org/10.1007/BF02319647
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DOI: https://doi.org/10.1007/BF02319647