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Preparative scale reversed-phase HPLC method for simultaneous separation of carotenoids and carotenoid esters

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Summary

A method is described for the preparative HPLC of carotenoids and carotenoid esters using a self-packing axially-compressed column. The reversed-phase system used employs an RP-18 stationary phase and a mobile phase consisting of a mixture of petroleum spirit, acetonitrile, methanol and tetrahydrofuran. The method is demonstrated for paprika fruit, rose hips and marigold flowers.

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Authors and Affiliations

  1. Department of Chemistry, University of Bergen, Allegaten 41, N-5007, Bergen, Norway

    S. Scalia & G. W. Francis

  2. Department of Pharmaceutical Sciences, University of Ferrara, I-44100, Ferrara, Italy

    S. Scalia

Authors
  1. S. Scalia
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  2. G. W. Francis
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Scalia, S., Francis, G.W. Preparative scale reversed-phase HPLC method for simultaneous separation of carotenoids and carotenoid esters. Chromatographia 28, 129–132 (1989). https://doi.org/10.1007/BF02319633

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  • DOI: https://doi.org/10.1007/BF02319633

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