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High pressure liquid chromatographic determination with ultraviolet detection of gentamicin and correlation with the microbiological potency

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Abstract

The content of gentamicin in pharmaceuticals is measured by the high pressure liquid Chromatographic technique described in the monographs for Gentamicin Sulphate and Gentamicin Injection in the British Pharmacopoeia 1980 for the determination of the composition of gentamicin. Calculation procedures are presented to transform this qualitative high pressure liquid Chromatographic procedure for the assay of gentamicin so that it gives results in terms of potency. A comparison is made of this data with the results of the microbiological assay according to the European Pharmacopoeia.

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Authors and Affiliations

  1. National Institute for Quality Control of Drugs (RIGO), W. Barentszstraat 7, 2315, TZ Leiden, the Netherlands

    J. W. van Duijn, D. M. Barends & D. W. Groot

  2. Laboratory for Chemotherapy, National Institute of Public Health and Environmental Protection (RIV), P.O. Box I, 3720, BA Bilthoven, the Netherlands

    A. J. de Neeling

Authors
  1. J. W. van Duijn
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  2. D. M. Barends
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  3. D. W. Groot
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  4. A. J. de Neeling
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Additional information

On the occasion of the twenty-fifth anniversary of RIGO (director: H.M. Smits)

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van Duijn, J.W., Barends, D.M., Groot, D.W. et al. High pressure liquid chromatographic determination with ultraviolet detection of gentamicin and correlation with the microbiological potency. Pharmaceutisch Weekblad Scientific Edition 10, 267–271 (1988). https://doi.org/10.1007/BF02219666

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  • DOI: https://doi.org/10.1007/BF02219666

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