Abstract
Purpose
The administration of two GnRH analogues (buserelin and leuprolide acetate) in long and short protocols was evaluated prospectively. In the long protocol, the analogues were given from day 1 of the cycle for at least 14 days and then hMG administration was started, while in the short protocol the analogues were initiated from cycle day 1, adding hMG from day 3. The patients were divided into four groups according to the protocol used: Group I, buserelin-long; Group II, buserelin-short; Group III, leuprolide-long; and Group IV, leuprolide-short. Serum E 2 levels on the day of hCG injection and the number of follicles observed, oocytes retrieved, and embryos obtained, as well as implantation rates, were significantly higher (P<0.001) in the long protocols than in the short ones.
Results
Pregnancy rates were similar in all groups, although a trend for better results was observed in buserelin-long compared to buserelin-short. There were no differences in the results achieved with buserelin or leuprolide.
Conclusion
The administration of GnRH analogues (buserelin and leuprolide acetate) in long protocols induced a more intense ovarian response and was associated with significantly higher implantation rates and also a trend for higher pregnancy rates, although this difference was not statistically significant.
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References
Smitz J, Ron-El R, Tarlatzis BC: The use of gonadotrophin releasing hormone agonists for in vitro fertilization and other assisted procreation techniques: Experience from three centers. Hum Reprod 1992;7(Suppl 1):49–66
Zeevi D, Younis SJ, Laufer N: Ovulation induction—New approaches. Assist Reprod Rev 1991;1:2–8
Testart J, Plachot M, Mandelbaum J, Salat-Baroux J, Frydman R, Cohen J: World collaborative report on IVF-ET and GIFT: 1989 results. Hum Reprod 1992;7:362–369
Testart J, Lefevre B, Gougeon A: Effects of gonadotrophinreleasing hormone agonists (GnRH-a) on follicle and oocyte quality. Hum Reprod 1993;8:511–518
Barrière P, Lopes P, Boiffart J-P, Pousset C, Quentin M, Sagot P, L'Hermite A, Levat MF, Charbonnel B: Use of GnRH analogues in ovulation induction for in vitro fertilization. Benefits of a short administration regimen. J Vitro Fertil Embryo Transfer 1987;4:64–65
Smitz J, Devroey P, Broekmans P, Camus M, Khan I, Staessen C, Van Waesberghe L, Wisanto A, Van Steirteghem AC: Management of failed cycles in an IVF/GIFT programme with the combination of a GnRH analog and hMG. Hum Reprod 1987;2:309–314
Tarlatzis BC, Bili H: LH-RH analogues and follicular fluid in IVF cycles. Releaser 1992;7(2):3–7
Latouche J, Coumeyrolle-Arias M, Jordan D, Kopp N, Augendre-Ferrante B, Cedard L, Haour F: GnRH receptors in human granulosa cells: Anatomical localization and characterization by autoradiographic study. Endocrinology 1989;125:1739–1741
Parinaud J, Beaur A, Bourreau M, Vieitez G, Pontonnier G: Effect of a LHRH agonist (buserelin) on steroidogenesis of cultured human preovulatory granulosa cells. Fertil Steril 1988;50:597–602
Bramley T, Menzies G, Baird D: Specificity of GnRH binding sites of human corpus luteum: Comparison with receptors of rat pituitary gland. J Endocrinol 1986;108:323–328
Tarlatzis BC, Pados G, Bontis J, Lagos S, Grimbizis G, Spanos E, Mantalenakis S: Ovarian stimulation with Buserelin/ hMG/hCG: Prospective randomised study of short versus long protocol. Hum Reprod 1993;8:807–812
Tarlatzis BC, Grimbizis G: Assisted reproduction techniques in polycystic ovarian syndrome. Ann NY Acad Sci 1993;687:280–287
Tarlatzis BC: Oocyte collection and quality. Assist Reprod Rev 1992;2:16–22
Hedon B, Arnal F, Basor E, Boulot P, Huet JM, Fries N, Deschamps F, Cristal P, Humeau C: Comparaison randomisée protocole long-protocole court dans les stimulations de l'ovaire en association avec un agoniste de la GnRH en vue de fecondation in vitro. Contracept Fertil Sexual 1988;16:624–627
Tan SL, Kingsland C, Cambell S, Mills C, Bradfield J, Alexander N, Yovich J, Jacobs HS: The long protocol of administration of gonadotropin-releasing hormone agonist is superior to the short protocol for ovarian stimulation for in vitro fertilization. Fertil Steril 1992;57:810–814
Remorgida V, Anserini P, Crose S, Costa M, Ferrailo A, Centonze A, Gaggero G, Capitanio G: The duration of pituitary suppression by means of intranasal gonadotropin-releasing hormone administration does not influence the ovarian response to gonadotropin stimulation and success rate in a gamete intrafallopian transfer (GIFT) program. J Vitro Fertil Embryo Transfer 1989;6:76–80
Frydman R, Parneix I, Bellaish-Allart J, Forman R, Hazout A, Fernandez H, Testart J: LHRH agonists in IVF: different methods of utilization and comparison with previous ovulation stimulation treatments. Hum Reprod 1988;3:559–561
Acharya U, Small J, Randall J, Hamilton M, Templeton A: Prospective study of short and long regimens of gonadotropin-releasing hormone agonist in in vitro fertilization program. Fertil Steril 1992;57:815–818
Meldrum D, Steingold K, de Ziegler D, Chetkowski R, Hamilton F, Randle D: Influence of the resting state of the ovary on recruitment of multiple follicles for IVF/ET. 4th World Conference on in Vitro Fertilization, Melbourne, Australia, 1985, abstr 188
Loumaye E, Vankrieten L, Depreester S, Psalti I, de Cooman S, Thomas K: Hormonal changes induced by short-term administration of a gonadotropin-releasing hormone agonist during ovarian hyperstimulation for in vitro fertilization and their consequences for embryo development. Fertil Steril 1989;51:105–111
Parinaud J, Oustry P, Perineau M, Rème J-M, Monroziès X, Pontonnier G: Randomized trial of three luteinizing hormone-releasing hormone analogues used for ovarian stimulation in an in vitro fertilization program. Fertil Steril 1992;57:1265–1268
Balasch J, Jové CI, Moreno V, Civico S, Puerto B, Vanvell AJ: The comparison of two gonadotropin-releasing hormone agonists in an in vitro fertilization program. Fertil Steril 1992;58:991–994
Testart J, Forman R, Bellaish-Allart J, Volante M, Hazout A, Strubb N, Frydman R: Embryo quality and uterine receptivity in in-vitro fertilization cycles with or without agonists of gonadotropin releasing hormone. Hum Reprod 1989;4:198–202
Brzyski GR, Jones GS, Jones WH, Oehninger S, Muasher JS: Alterations in luteal phase progesterone and estradiol production after leuprolide acetate therapy before ovarian stimulation for in vitro fertilization. Fertil Steril 1991;55:119–124
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Tarlatzis, B.C., Grimbizis, G., Pournaropoulos, F. et al. Evaluation of two gonadotropin-releasing hormone (GnRH) analogues (leuprolide and buserelin) in short and long protocols for assisted reproduction techniques. J Assist Reprod Genet 11, 85–91 (1994). https://doi.org/10.1007/BF02215993
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DOI: https://doi.org/10.1007/BF02215993