Skip to main content
SpringerLink
Log in

Platelet activating factor treatment of spermatozoa enhances rabbit oocyte fertilization following subzonal sperm insertion

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The objective of this study was to evaluate the effects of spermatozoal platelet activating factor (PAF) treatment on fertilization rates employing subzonal sperm insertion in the rabbit.

Study Design

Oocytes (n=130) with distinct first polar bodies were injected with three to seven motile spermatozoa. Half (n=65) of the oocytes were injected with PAF-treated sperm, whereas the other half (n=65) served as controls and were injected with nontreated sperm. Fertilization rates were assessed by the formation of two pronuclei followed by cleavage to the two-cell stage.

Results

In the group of oocytes injected with PAF-treated sperm, we noted a significant (P<0.001) improvement in fertilization rates, 61.5% (40/65), vs. control nontreated sperm, 20.0% (13/65). Subsequent cleavage to the two-cell stage was noted for 95% (38/40) in the treated vs 92.3% (12/13) in the nontreated fertilized zygotes.

Conclusion

We conclude that PAF treatment of rabbit spermatozoa prior to subzonal insertion improves fertilization rates and may be of clinical significance in assisted reproductive programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Harper MJK: Platelet activating factor: A paracrine factor in preimplantation stages of development? Biol Reprod 1989;40:907–913

    PubMed  Google Scholar 

  2. Minhas BS, Kumar R, Ricker DD, Robertson JL, Dodson MG: The presence of platelet activating factor-like activity in human spermatozoa. Fertil Steril 1991;55:372–376

    PubMed  Google Scholar 

  3. Kumar R, Harper MJK, Hanahan DJ: Occurrence of platelet-activating factor in rabbit spermatozoa. Arch Biochem Biophys 1988;360:497–502

    Article  Google Scholar 

  4. Parks JE, Hough S, Elrod C: Platelet activating factor activity in the phospholipids of bovine spermatozoa. Biol Reprod 1990;43:806–811

    PubMed  Google Scholar 

  5. Kuzan FB, Geissler FT, Henderson WR: Role of platelet activating factor (PAF) in mouse in vitro fertilization. Prostaglandins 1990;39:61–74

    Article  PubMed  Google Scholar 

  6. Minhas BS: Platelet activating factor and its role in mammalian fertilization.In Advances in Physiological Sciences, SK Manchanda, W Selvamurthy, V Mohan Kumar (eds). New Delhi, Macmillan Press, 1992, pp 472–482

    Google Scholar 

  7. Shi H, Miller M, Miller K, Kim MH: The effect of platelet activating factor on different phases of murine in vitro fertilization. J Assist Reprod Genet 1992;9:373–377

    Article  PubMed  Google Scholar 

  8. Minhas BS, Kumar R, Ricker DD, Roudebush WE, Dodson MG, Fortunato SJ: The effects of platelet activating factor on mouse oocyte fertilization in vitro. Am J Obstet Gynecol 1989;161:1714–1717

    PubMed  Google Scholar 

  9. Roudebush WE, Minhas BS, Ricker DD, Palmer TV, Dodson MG: Platelet activating factor enhances in vitro fertilization of rabbit oocytes. Am J Obstet Gynecol 1990;163:1670–1673

    PubMed  Google Scholar 

  10. Sengoku K, Ishikawa M, Tamate K, Shimizu T: Effects of platelet activating factor on mouse sperm function. J Assist Reprod Genet 1992;9:447–453

    Article  PubMed  Google Scholar 

  11. Ricker DD, Minhas BS, Kumar R, Robertson JL, Dodson MG: The effects of platelet activating factor on the motility of human spermatozoa. Fertil Steril 1989;52:655–658

    PubMed  Google Scholar 

  12. Hellstrom WJG, Wang R, Sikka SC: Platelet-activating factor stimulates motion parameters of cryopreserved human sperm. Fertil Steril 1991;56:768–770

    PubMed  Google Scholar 

  13. Jarvi K, Roberts KD, Langlais J, Gagnon C: Effect of platelet-activating factor, lyso-platelet activating factor, and lysophosphatidyl-choline on sperm motion: Importance of albumin for motility stimulation. Fertil Steril 1993;59:1266–1275

    PubMed  Google Scholar 

  14. Angle MJ, Librach CL, Tom R, McClure D: The effect of platelet activating factor on sperm-mucus interaction.In Proceedings of the Forty-Fifth Annual Meeting of the American Fertility Society, San Francisco, CA, Nov 13–16, 1989, p S98 (abstr P-091)

  15. Minhas BS: Platelet activating factor treatment of human spermatozoa enhances fertilization potential. Am J Obstet Gynecol 1993;168:1314–1317

    PubMed  Google Scholar 

  16. Minhas BS, Roudebush WE, Ricker DD, Dodson MG: Treatment of sperm with high ionic strength medium increases microsurgical fertilization rates of rabbit oocytes fertilized by subzonal placement of sperm. J Vitro Fert Embryo Transfer 1991;8:111–115

    Article  Google Scholar 

  17. Braquet P, Touqui L, Shen TY, Vargaftig BB: Perspectives in platelet activating factor research. Pharmacol Rev 1987;39:97–145

    PubMed  Google Scholar 

  18. Sengoku K, Tamate K, Takaoka Y, Ishikawa M: Effects of platelet activating factor on human sperm function in vitro. Hum Reprod 1993;8:1443–1447

    PubMed  Google Scholar 

  19. Fukuda A, Roudebush WE, Thatcher SS: Platelet activating factor enhances the acrosome reaction, fertilization in vitro by sperm injection and resulting embryonic development in the rabbit. Hum Reprod 1994;9:94–99

    PubMed  Google Scholar 

  20. Oehninger S, Sueldo C, Burkman LJ, Mahoney M, Alexander NJ: Effect of progesterone on human sperm functions: hyperactivated motility, acrosome reaction, zona pellucida binding and oocyte penetration. 48th Annual Meeting, American Fertility Society, New Orleans, LA, Nov 2–5, 1992, p S181 (abstr P266)

    Google Scholar 

  21. Minhas BS, Zhu YP, Kim HN, Ripps BA, Buster JE: Platelet activating factor content and release from rabbit spermatozoa is stimulated by progesterone treatment. 40th Annual Meeting, Society for Gynecologic Investigation, Toronto, Canada, Mar 31–Apr 3, 1993, p 357 (abstr P349)

    Google Scholar 

  22. Ripps BA, Zhu YP, Kim HN, Buster JE, Carson SA, Minhas BS: Progesterone treatment stimulates human spermatozoal platelet activating factor. American Fertility Society conjointly with the Canadian Fertility and Andrology Society, Montreal, Canada, Oct 11–14, 1993, p S77 (abstr 0159)

    Google Scholar 

  23. O'Neill C, Ryan JP, Collier M, Saunders DM, Ammit AJ, Pike IL: Outcome of pregnancies resulting of a trial of supplementing human IVF culture medium with platelet activating factor. Reprod Fert Dev 1992;4:109–112

    Article  Google Scholar 

  24. Minhas BS: Platelet activating factor treatment of spermatozoa does not impair embryonic development in vitro or in vivo. Mol Androl 1992;3:1–7

    Google Scholar 

Download references

Author information

Author notes

  1. Hak -Nam Kim

    Present address: Fertility Center of New England, 01867, Reading, Massachusetts

  2. Barry A. Ripps

    Present address: Department of Obstetrics and Gynecology, University of Florida, 32503, Pensacola, Florida

  3. Thomas H. Burwinkel

    Present address: Department of Obstetrics and Gynecology, Wright State University, 45409, Dayton, Ohio

Authors and Affiliations

  1. Department of Obstetrics & Gynecology, The University of Tennessee, Memphis, Tennessee

    Brijinder S. Minhas, Hak -Nam Kim, Yan -Ping Zhu, Barry A. Ripps & Thomas H. Burwinkel

  2. Center for Human Reproduction, 750 North Orleans Street, 60610, Chicago, Illinois

    Brijinder S. Minhas & Yan -Ping Zhu

Authors
  1. Brijinder S. Minhas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hak -Nam Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan -Ping Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Barry A. Ripps
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas H. Burwinkel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Minhas, B.S., Kim, H.N., Zhu, Y.P. et al. Platelet activating factor treatment of spermatozoa enhances rabbit oocyte fertilization following subzonal sperm insertion. J Assist Reprod Genet 11, 308–311 (1994). https://doi.org/10.1007/BF02215718

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02215718

Key words

Search

Navigation