Abstract
Purpose
Assisted reproductive technologies require the use of culture media for gametes and embryos. Culture media and conditions intended for use in human in vitro fertilization are tested prior to use by evaluating their ability to support normal development of mouse embryos.
Results
Development to blastocysts of two-cell mouse embryos was equivalent for embryos cultured in center well dishes alone (93±10%, mean±SD) and embryos cultured in center well dishes with an overlay of 1 ml of mineral oil (94±5%). In contrast, no development of two-cell-stage mouse embryos occurred in an embryotoxic medium when cultured in center well dishes. However, when this medium was covered with an overlay of mineral oil, development to blastocyst was no different than that of controls (96±5%). Similar results were found with cultures of one-cell-stage mouse embryos.
Conclusion
These results indicate that in some circumstances the presence of mineral oil can significantly alter the outcome of embryo culture. Further, these results reinforce the necessity of directly testing the exact culture conditions to be used for assisted reproductive technologies.
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References
Brinster RL: A method for in vitro cultivation of mouse ova from two-cell to blastocyst. Exp Cell Res 1963;32:205–208
SAS Institute Inc: SAS User's Guide: Statistics, Version 5 Edition. Cary NC: SAS Institute Inc, 1985
Quinn P, Warnes GM, Kerin JF, Kirby C: Culture factors in relation to the success of human in vitro fertilization and embryo transfer. Fertil Steril 1984;41:202–209
Gorrill MJ, Rinehart JS, Tamhane AC, Gerrity M: Comparison of the hamster sperm motility assay to the mouse one-cell and two-cell embryo bioassays as quality control tests for in vitro fertilization. Fertil Steril 1991;55:345–354
Beyler SA, Chong AP: In vitro fertilization culture medium surveys. Arch Pathol Lab Med 1992;116:425–429
Miller KF, Pursel VG: Absorption of compounds in medium by the oil covering microdrop cultures. Gamete Res 1987;17:57–61
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Miller, K.F., Goldberg, J.M. & Collins, R.L. Covering embryo cultures with mineral oil alters embryo growth by acting as a sink for an embryotoxic substance. J Assist Reprod Genet 11, 342–345 (1994). https://doi.org/10.1007/BF02214139
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DOI: https://doi.org/10.1007/BF02214139