Abstract
Purpose
The objective of this study was to investigate the influence of thyroid hormone on estradiol and progesterone secretion of human granulosa cells maintained in vitro.
Methods
Granulosa cells were obtained by aspiration of preovulatory follicles of woman undergoing assisted reproductive technology. Ovulation induction was performed with GnRH agonist, hMG, and hCG.
Results
Granulosa cells were maintained in vitro in a defined medium with added insulin. Twenty-four-hour estradiol and progesterone secretion into the medium were determined for granulosa cells growing in serum-free medium and in serum-free medium with added T4 in a concentration range of 10−7 to 10−11 M.
Conclusions
All concentrations of T4 used produced a statistically significant increase in progesterone secretion (range, 1.39 to 1.60 times the baseline amount). The increase in estradiol secretion reached statistical significance only at a T4 concentration of 10−8 M (1.24 times the baseline amount).
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References
Cunningham FG, MacDonald PC, Grant NF: Williams Obstetrics, 18th ed. Norwalk, CT, Appleton and Lange, 1989, pp 822–825
Channing CP, Tsai V, Sachs D: Role of insulin, thyroxine and Cortisol in luteinization of porcine granulosa cells grown in chemically defined media. Biol Reprod 1976;15:235–240
Wakim NG, Ramani N, Rao ChV: Triiodothyronine receptors in porcine granulosa cells. Am J Obstet Gynecol 1987;156:237–240
Maruo T, Hiramatusu S, Otani T, Hayaski M, Mochizuki M: Increase in the expression of thyroid hormone receptors in porcine granulosa cells early in follicular maturation. Acta Endocrinol (Copenh) 1992;127:152–160
Maruo T, Hayashi M, Matsho H, Yamamoto T, Okada H, Mochizuki M: The role of thyroid hormone as a biological amplifier of the actions of follicle stimulating hormone in the functional differentiation of cultured porcine granulosa cells. Endocrinology 1987;121:1233–1241
Biswas R, Bandyopadhyay A, Guin S, Bhattacharya S: Binding of thyroid hormone to mouse granulosa cell nuclei and its biological relevance. J Biosci 1993;18:327–335
Wakim AN, Polizotto SL, Buffo MJ, Marrero MA, Burholt DR: Thyroid hormones in human follicular fluid and thyroid hormone receptors in human granulosa cells. Fertil Steril 1993;59:1187–1190
Wakim AN, Polizotto SL, Burholt DR: Alpha 1 and beta 1 thyroid hormone receptors on human granulosa cells. Recent Prog Hormone Res 1994;49:377–381
Wakim AN, Paljug WR, Jasnosz KM, Alhakim N, Brown AB, Burholt DR: Thyroid hormone receptor messenger ribonucleic acid in human granulosa and ovarian stromal cells. Fertil Steril 1994;62:3;531–534
Dain LB, Stein P, Krimer ARD, Asch RH, de Fried EP, Charreau EH, Tensone M: Progesterone production in cultured human granulosa cells: Correlation with follicular fluid hormone levels. Fertil Steril 1991;55:1093–1098
Bodis J, Torok A, Tinneberg HR, Hanf VH, Hamori M, Cledon P: Influence of serotonin on progesterone and estradiol secretion of cultured human granulosa cells. Fertil Steril 1992;57:1008–1011
Mag JV, Schomberg DW: Granulosa cell differentiation in vitro: Effect of insulin and functional integrity. Biol Reprod 1981;25:421–424
Davoren JB, Hsueh AJW: Insulin enhances FSH-stimulated steroidogenesis by cultured rate granulosa cells. Mol Cell Endocrinol 1984;35:97–105
Goldman S, Dirnfeld M, Abramovici H, Kraiem Z: Triiodo-thyronine (T3) modulates hCG-regulated progesterone secretion, cAMP accumulation and DNA content in cultured human luteinized granulosa cells. Mol Cell Endocrinol 1993;96:125–131
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Wakim, A.N., Polizotto, S.L. & Burholt, D.R. Influence of thyroxine on human granulosa cell steroidogenesis in vitro. J Assist Reprod Genet 12, 274–277 (1995). https://doi.org/10.1007/BF02212931
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DOI: https://doi.org/10.1007/BF02212931