Abstract
Purpose
The effects of a GnRH agonist (GnRHa) on oocyte quality were investigated by assessing the influence of GnRHa on oocytes, and fertilized oocytes were examined in vivo and in vitro. Administration of gonadotropin in conjunction with GnRHa induced a significantly greater degree of germinal vesicle breakdown, significantly higher rates of in vitro fertilization, and significantly faster development of the oocytes than with pregnant mare serum gonadotropin alone.
Results
The hatching-success rate in the GnRHa treated hypophysectomized mice was higher than in control mice. The rate of in vitro fertilization was also higher in oocytes cultured in the presence of low doses of GnRHa and these effects were reversed by a GnRH antagonist.
Conclusion
Oocytes obtained following ovarian stimulation with GnRHa were of higher quality than control oocytes, and the efficacy of GnRHa may be due in part to its direct action on the ovary.
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References
Johnson P, Pearce JM: Recurrent spontaneous abortion and polycystic ovarian disease: Comparison of two regimens to induce ovulation. Br Med J 1990;300:154–156
Leung PCK, Steele GL: Intracellular signaling in the gonads. Endocrine Rev 1992;13:476–489
Uemura T, Yanagisawa T, Shirasu K, Matsuyama A, Minaguchi H: Mechanisms involved in the pituitary desensitization induced by gonadotropin-releasing hormone agonists. Am J Obstet Gynecol 1992;167:283–291
Quinn P, Kerin JF, Warnes GM: Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985;44:493–498
Vauthier D, Lefebvre G: The use of gonadotropin-releasing hormone analog for in vitro fertilization: Comparison between the standard form and long-acting formulation of D-Trp-6-luteinizing hormone releasing hormone. Fertil Steril 1989;51:100–104
Palermo R, Amodeo G, Navot D, Rosenwaks Z, Citttadini E: Concomitant gonadotropin-releasing hormone agonist and menotropin treatment for the synchronized induction of multiple follicles. Fertil Steril 1988;49:290–295
Keenan D, Cohen J, Suzman M: Stimulation cycles suppressed with gonadotropin-releasing hormone analog yield accelerated embryos. Fertil Steril 1991;55:792–796
Hsueh A, Erickson G: Extrapituitary actions of gonadotropin-releasing hormone; direct inhibition of ovarian steroidogenesis. Science 1979;204:854–855
Yoshimura Y, Ubukata Y, Nakamura Y, Ando M, Yamada Y, Suzuki M, Nanno T: Gonadotropin-releasing hormone agonists induce meiotic maturation and degeneration of oocytes in the in vitro perfused rabbit ovary. Fertil Steril 1991;55:170–183
Hseuh AJW, Jones PBC: Extrapituitary actions of gonadotropin-releasing hormone. Endocrine Rev 1981;2:437–461
Wang C, Leung A: LHRH stimulates plasminogen activator and inhibits steroid production by granulosa cells of adult rat graafian follicles. Mol Cell Endocrinol 1986;44:61–68
Rante T, Knecht M, Baukal AJ, Korhonen M, Catt KJ: GnRH agonist-induced inhibitory and stimulatory effects during ovarian follicular maturation. Mol Cell Endocrinol 1984;35:55–63
Parinaud J, Vieitez G, Beaur A, Pontonnier G, Bourreau E: Effect of a luteinizing hormone-releasing hormone agonist (Buserelin*) on steroidogenesis of cultured human preovulatory granulosa cells. Fertil Steril 1988;50:597–602
Dekel N, Lewysohn O, Ayalon D, Hazum E: Receptors for gonadotropin releasing hormone are present in rat oocytes. Endocrinology 1988;123:1205–1207
Wang J, Lee V, Leung P: Differential role of protein kinase C in the action of luteinizing hormone-releasing hormone on hormone production in rat ovarian cells. Am J Obstet Gynecol 1989;160:984–989
Wang J, Leung P: Arachidonic acid as a stimulatory mediator of luteinizing hormone-releasing hormone action in the rat ovary. Endocrinology 1989;124:1973–1979
Latouche J, Crumeyrolle-Arias M, Jordan D, Kopp N, Augendre-Ferrante B, Cedard L, Haour F: GnRH receptors in human granulosa cells: anatomical localization and characterization by autoradiographic study. Endocrinology 1989;125:1739–1741
Kakar SS, Musgrove LC, Devor DC, Sellers JC, Neill JD: Cloning, sequencing and expression of human gonadotropin releasing hormone (GnRH) receptor. Biochem Biophys Res Commun 1992;189:289–295
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Yang, B.C., Uemura, T. & Minaguchi, H. Effects of a gonadotropin releasing hormone agonist on oocyte maturation, fertilization, and embryonal development in mice. J Assist Reprod Genet 12, 728–732 (1995). https://doi.org/10.1007/BF02212901
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DOI: https://doi.org/10.1007/BF02212901