Abstract
Purpose
The aim of our study is to elucidate whether human oocyteslembryos secrete IFN γ and/or IL-10 and whether the fertilization process depends on the balance between these cytokines.
Methods
A total of 142 embryo culture media from 24 patients were collected and the cytokine levels were tested with ELISA.
Results
IFN γ and IL-10 were detectable in 40.1% and 29.6% of culture media respectively. The difference of IFN γ and IL-10 levels in media from fertilized oocytes between day 1 and day 2 are significant (0.46 vs. 1.47 and 34.2 vs. 12.7, respectively). However there was no significant difference between the IFN γ levels of the media from fertilized and nonfertilized oocytes 0.46 vs. 0.85 at day 1 and 1.47 vs. 1.49 at day 2, as well as IL-10 levels 34.2 vs. 30.9 at day 1 and 12.7 vs. 9.58 at day 2 respectively.
Conclusions
Human preimplantation embryos secrete the cytokines IFN γ and IL-10. No effect of these cytokines on fertilization process could be shown.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL: Two types of murine helper T cell clone I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 1986;136:2348–2357
Mosmann TR: Properties and functions of interleukin-10. Adv Immunol 1994;56:1–26
Wegmann TG, Lin H, Guilbert L, Mosmann TR: Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a Th2 phenomenon? Immunol Today 1993;14:353–356
Lin H, Mosmann TR, Guilbert L, Tuntipopipat S, Wegmann TG: Synthesis of T helper 2-type cytokines at the maternal-fetal interface. J Immunol 1993;151:4562–4573
Jones KP, Warnock SH, Urry RL, Edwin SS, Mitchell MD: Immunosuppressive activity and alpha interferon concentrations in human embryo culture media as an index of potential for successful implantation. Fertil Steril 1992;57:637–640
Zolti M, Ben-Rafael Z, Meriom R, Shemesh M, Bider D, Mashiach S, Apte RN: Cytokine involvement in oocytes and early embryos. Fertil Steril 1991;56:265–272
Austgulen R, Arntzen KJ, Vatten LJ, Kahn J, Sunde A: Detection of cytokines (interleukin-1, interleukin-6, transforming growth factor-Β) and soluble tumour necrosis factor receptors in embryo culture fluid during in-vitro fertilization. Hum Reprod 1995;10:171–176
DeMaeyer E, DeMaeyer-Guignard J: Interferons.In The Cytokine Handbook, A Thomson (ed). London, Academic Press, 1992, pp 215–240
La Bonnardiere C: Nature and possible functions of interferons secreted by the preimplantation pig blastocyst. J Reprod Fertil Suppl 1993;48:157–170
La Bonnardiere C: No detection of interferon gamma activity during early pregnancy in the mouse. Am J Reprod Immunol 1993;30:26–31
Feinman MA, Klinman HJ, Main EK: HLA antigen expression and induction by γ-interferon in human trophoblasts. Am J Obstet Gynecol 1987;157:1429–1434
Kovats S, Main EK, Librach C, Stubblebine M, Fisher SJ, DeMars R: A class I antigen, HLA-G, expressed in human trophoblast. Science 1990;248:220–223
Loke YW, King A: Recent developments in the human maternal-fetal immune interaction. Curr Opin Immunol 1991;3:762
Moore KW, O'Garra A, Malefyt RW, Vieira P, Mosmann TR: Interleukin-10. Annu Rev Immunol 1993;11:165–190
Danzer SG, Kirchner H, Rink I: Cytokine interactions in human mixed lymphocyte culture. Transplantation 1994;57:1638–1642
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
özörnek, M.H., Bielfeld, P., Krüssel, J.S. et al. Interferon gamma and interleukin 10 levels in preimplantation embryo culture media. J Assist Reprod Genet 12, 590–593 (1995). https://doi.org/10.1007/BF02212580
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02212580