Abstract
Purpose
To evaluate biochemically and clinically a new formulation of modified Ham's F-10 medium made without the inclusion of hypoxanthine. The medium was formulated for long-term storage and use by separately preparing a stable liquid (“basal”) portion and a freeze-dried “supplement” containing the labile medium components.
Results
Following 18 months of storage the basal medium was biochemically analyzed for its amino acid (aa's) and vitamin content. Cysteine and tryptophan were decreased to less than 30% of their starting theoretical concentrations (STCs). Asparagine, serine, tyrosine, histidine and lysine were present at 50% to 70% of their STC. The remaining aa's were all within 90% of their STCs except arginine which was at 77%. All of the vitamins were present at 90% or more of their STCs except inositol, riboflavin and'thiamine which were present at 70% of their STCs. IVF with the new formulation resulted in 13 deliveries from 51 aspirations (25%) as compared with 10/39 (26%) in 1991, when standard medium preparation was used. Oocyte donation resulted in 30 deliveries from 84 cycles (36%) with the new formulation as compared with 21/65 (32%) in 1991.
Conclusions
(1) The new basal with lyophilized supplement formulation produces similar clinical results in the IVF laboratory as medium prepared in the standard fashion, (2) certain amino acids and vitamins are not stable in the liquid basal medium, and (3) the separate formulation of a liquid basal medium with lyophilized supplement is convenient, viable alternative to modified Ham's F-10 medium prepared in the standard manner (i.e., from powder) and may decrease the need for frequent medium preparation.
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References
Purdy JM: Methods for fertilization and embryo culture in vitro.In: Human Conception in Vitro, RG Edwards, JM Purdy (eds). London, Academic Press, 1982, pp135–148
Wales RG, Whittingham DG: Decomposition of sodium pyruvate in culture media stored at 5 C and its effects on the development of the preimplantation mouse embryo. J Reprod Fertil 1971;24:126 (abstract)
Bernart W, Hauff B, Malez-Kehry W, Kunz G, Leyendecker G: Frozen storage of Ham's F-10 medium for human in-vitro fertilization. Hum Reprod 1990;5:610–612
Leung PCS, Gronow MJ, Kellow GN, Lopata A, Spiers AL, du Plessis YP, Johnson I: Serum supplement in human in vitro fertilization an embryo development. Fertil Steril 1984;41:36–39
Naz RK, Janousek JT, Moody T, Stillman RJ: Factors influencing murine embryo bioassay: effects of proteins, aging of the medium, and surgical glove coatings. Fertil Steril 1986;46:914–919
Meldrum DR, Chetkowski R, Steingold KA, de Zieger D, Cedars M, Hamilton M: Evolution of a highly successful in vitro fertilization-embryo transfer program. Fertil Steril 1987;48:86–93
Davidson A, Vermesh M, Lobo RA, Paulson RJ: Mouse embryo culture as quality control for human in vitro fertilization: the one-cell versus the two-cell model. Fertil Steril 1988;49:516–521
Paulson RJ: In vitro fertilization and other assisted reproductive techniques. J Reprod Med 1993;38:261–268
Paulson RJ, Sauer MV, Lobo RA: Embryo implantation following human in vitro fertilization: Importance of endometrial receptivity. Fertil Steril 1990;53:870–874
Sauer MV, Paulson RJ, Macaso TM, Francis-Hernandez M, Lobo R: Establishment of a nonanonymous donor oocyte program: preliminary experience at the University of Southern California. Fertil Steril 1989;52:433–436
Sauer MV, Paulson RJ: Human oocyte and preembryo donation: An evolving method for the treatment of infertility. Am J Obstet Gynecol 1990;163:1421–1424
Sauer MV, Paulson RJ, Lobo RA: Reversing the natural decline in human fertility: An extended clinical trial of oocyte donation to women of advanced reproductive age. JAMA 1992;268:1275–1279
Chiu TTY, Tam PLP: A correlation of the outcome of clinical in vitro fertilization with the inositol content and embryotrophic properties of human serum. J Asst Reprod Genet 1992;9:524–530
Kane MT, Norris M, Harrison RAP: Uptake and incorporation of inositol by preimplantation mouse embryos. J Reprod Fertil 1992;96:617–625
Daniel JC, Jr: Vitamins and growth factors in the nutrition of rabbit blastocytes in vitro. Growth 1967;31:71–77
Bavister BD, Arlotto T: Influence of single amino acids on the development of hamster one-cell embryos in vitro. Molec Reprod Devel 1990;25:45–51
De Silva M: Effect of storage of Ham's F-10 medium on one-cell mouse embryo development in vitro. J Asst Reprod Genet 1993;10:238–241
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Modified Ham's F-10 Medium, Irvine Scientific, Santa Ana, California.
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Weathersbee, P.S., Francis, M.M., Macaso, T.M. et al. A new long shelf life formulation of modified Ham's F-10 medium: Biochemical and clinical evaluation. J Assist Reprod Genet 12, 175–179 (1995). https://doi.org/10.1007/BF02211794
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DOI: https://doi.org/10.1007/BF02211794