Skip to main content
Log in

Improvements in assessment of human failed fertilization oocytes using the supravital dye hoechst 33342

  • Short Communications
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Arndt-Jovin D, Jovin T: Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977;25:585–589

    PubMed  Google Scholar 

  2. Luttmer S, Longo F: Examination of living and fixed gametes and early embryos stained with supra vital fluorochromes (Hoechst 33342 and 3,3′-dihexyloxacarbocyanine Iodine). Gamete Res 1986;15:267–283

    Google Scholar 

  3. Latt SA, Stetten G: Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J Histochem Cytochem 1976;24:24–33

    PubMed  Google Scholar 

  4. Gwatkin RBL, Conover JC, Collins RL, Quigley MM: Failed fertilization in humanin vitro fertilization analyzed with the deoxyribonucleic acid-specific fluorochrome Hoechst 33342. Am J Obstet Gynecol 1989;160:31–35

    PubMed  Google Scholar 

  5. O'Rand MG, Herman B, Diguiseppi J, Halme J, Hammond MG, Talbert LM: Analysis of deoxyribonucleic acid distribution in non cleaving oocytes from patients undergoingin vitro fertilization. Fertil Steril 1986;46:452–460

    PubMed  Google Scholar 

  6. Schalkoff ME, Oskowitz SP, Powers RD: A multifactorial analysis of the pregnancy outcome in a successful embryo cryopreservation program. Fertil Steril 1993;59:1070–1074

    PubMed  Google Scholar 

  7. Van Wissen B, Bomsel-Helmreich O, Debey P, Eisenberg C, Vautier D, Pennehouat G: Fertilization and ageing processes in non-dividing human oocytes after GnRHa treatment: An analysis of individual oocytes. Human Reprod 1991;6:879–884

    Google Scholar 

  8. Pieters M, Dumoulin J, Englehart C, Bras M, Evers J, Geraedts J: Immaturity and aneuploidy in human oocytes after different stimulation protocols. Fertil Steril 1991;56:306–310

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Neuber, E., Witmyer, J., Oskowitz, S.P. et al. Improvements in assessment of human failed fertilization oocytes using the supravital dye hoechst 33342. J Assist Reprod Genet 11, 428–432 (1994). https://doi.org/10.1007/BF02211731

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02211731

Keywords

Navigation