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Colorimetric analysis of some diuretic drugs: hydrochlorothiazide and spironolactone

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Abstract

Two convenient spectrophotometric methods were developed for the determination of hydrochlorothiazide and spironolactone. A specific colour reaction for the determination of hydrochlorothiazide is reported. One method is based on the reaction of hydrochlorothiazide withn-butylamine and cobaltous chloride in anhydrous methanol. A blue-violet colour is produced and is measured at 570 nm. A highly selective colorimetric method was adopted for the analysis of spironolactone by reacting with isoniazid forming a coloured hydrazone derivative and subsequent measurement of the coloured product at 405 nm.

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Authors and Affiliations

  1. Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

    Bahia A. Moussa

  2. National Organization of Drug Control and Research, Cairo, Egypt

    Naglaa M. el Kousy

Authors
  1. Bahia A. Moussa
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  2. Naglaa M. el Kousy
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Additional information

From ‘Determination of certain diuretics’, M Pharm Sci thesis.

The volumetric flasks were dried by rinsing them with pure acetone after draining off the water, then allowing to drain until dry or blowing air through them.

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Moussa, B.A., el Kousy, N.M. Colorimetric analysis of some diuretic drugs: hydrochlorothiazide and spironolactone. Pharmaceutisch Weekblad Scientific Edition 7, 79–82 (1985). https://doi.org/10.1007/BF02106133

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  • DOI: https://doi.org/10.1007/BF02106133

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