Abstract
Purpose: The in vitro effect of anordrin and anordiol on the development of mouse two-cell embryos was studied.
Method: Female mice were primed with gonadotropins for superovulation and caged with male mice. Preimplantation embryos, at the two-cell stage, were recovered from the oviducts at 40 hr post-hCG. In the first experiment, two-cell embryos were exposed to culture medium containing different concentrations of anordrin for 3, 12, 24, and 80 hr and then grown in the anordrin-free culture medium and assessed for the formation of total and hatching blastocysts at 80 hr. In the second experiment, two-cell embryos were grown in culture medium containing different concentrations of anordiol and assessed for the formation of total and hatching blastocysts at 80 hr in vitro.
Results: Exposure of two-cell embryos to anordrin concentrations of 2.5–7.5µg/ml for 12 hr, 2.5–5.0µg/ml for 24 hr, and 2.5µg/ml for 80 hr caused significant inhibition of the formation of total blastocysts and to 2.5–7.5µg/ml for 12 hr, 1.0–2.5µg/ml for 24 hr, and 1.0µg/ml for 80 hr caused significant inhibition of the formation of hatching blastocysts, in a exposure time-dependent and dose-dependent manner. Exposure of two-cell embryos to anordiol concentrations of 15–25µg/ml for 80 hr caused significant inhibition of the formation of total blastocysts and to 15–20µg/ml for 80 hr caused significant inhibition of the formation of hatching blastocysts in a dose-dependent manner.
Conclusion: Anordrin and its metabolite anordiol inhibit the development of two-cell embryos in vitro.
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Juneja, S.C., Williams, R.S. Effect of anordrin on the development of mouse preimplantation embryos in vitro. J Assist Reprod Genet 13, 356–362 (1996). https://doi.org/10.1007/BF02070152
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DOI: https://doi.org/10.1007/BF02070152