Abstract
Purpose: Through problems in the management of embryo thawings and transfers, we investigated in a comparative study the use of the natural cycle and of a programmed short-term stimulation protocol as preparation method for the replacement of cryopreserved embryos. To date, 117 embryos have been thawed, and 60 (51%) survived with ≥ 50% intact blastomeres.
Methods: In 31 cases, replacement of the frozen-thawed embryos was planned in the natural cycle (group A), and in nine cases in the stimulated cycle (group B). In group A, 16 replacements could be performed (cancellation rate 48%). Six transfers took place on weekends (37%). Two clinical pregnancies could be established (13% per replacement). In group B, there were no canceled cycles (p<0.025). All thawings and transfers could be conducted between Monday and Friday. In one case, the single one frozen embryo was degenerated after thawing.
Results: Thus, eight replacements could be performed which resulted in three clinical pregnancies (38% per replacement, n.s.) and one biochemical pregnancy.
Conclusions: Based on our results, we conclude that a programmed short-term protocol not only provides viable embryos with a good cryopreservation potential, but it is also a reliable preparation method for the replacement of frozen-thawed embryos.
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References
Zorn JR, Boyer P, Guichard A: Never on a sunday: Programming for IVF-ET and GIFT. Lancet 1987;1:385–386
Imthurn B, Macas E, Hotz E, Keller PJ: Programmed stimulation for in vitro fertilization. Geburtsh Frauenheilk 1992;52:27–31
Macas E, Xie M, Keller PJ, Imthurn B, Rülicke T: Developmental capacities of two-cell mouse embryos frozen by three different methods. J Vitro Fert Embryo Transfer 1991;8:208–212
Cohen J, De Vane G, Elsner C, Fehilly C, Kort H, Massey J, Turner T: Cryopreservation of zygotes and early cleaved human embryos. Fertil Steril 1988;49:283–288
Freemann L, Trounson A, Kirby C: Cryopreservation of human embryos: progress on the clinical use of the technique in human in vitro fertilization. J Vitro Fert Embryo Transfer 1986;3:53–61
Macas E, Floersheim Y, Hotz E, Imthurn B, Keller PJ, Walt H: Abnormal chromosomal arrangements in human oocytes. Hum Reprod 1990;5:703–707
Lassalle B, Testard J, Renard JP: Human embryo features that influence the success of cryopreservation with the use of 1,2-propanediol. Fertil Steril 1985;44:645–651
Sathanandan M, Macnamee MC, Rainsbury P, Wick K, Brinsden P, Edwards RG: Replacement of frozen-thawed embryos in artificial and natural cycles: A prospective semi-randomized study. Hum Reprod 1991;6:685–687
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Imthurn, B., Macas, E., Rosselli, M. et al. Effect of a programmed short-term stimulation protocol on the replacement of cryopreserved embryos. J Assist Reprod Genet 13, 709–712 (1996). https://doi.org/10.1007/BF02066423
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DOI: https://doi.org/10.1007/BF02066423