Abstract
Purpose: Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes.
Method: A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser drilled oocytes.
Results: Fertilization was achieved only when three to five cattle sperm were trapped with optical tweezers and inserted directly through the laser drilled hole into the perivitelline space. After 20 hr, 3 of 79 (3.8%) oocytes revealed two pronuclei and a sperm tail within their cytoplasm. Cattle sperm are difficult to catch. Therefore, the gametes had to remain for about 20 min in room atmosphere, which might be the reason for the low fertilization results.
Conclusions: The results indicate that a combined UV-laser microbeam and optical tweezers trap can be used successfully for “noncontact” microinsemination procedures.
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References
Schütze K, Clement-Sengewald A: Catch and move—cut or fuse. Nature 1994;368:667–669
Tadir Y, Wright WH, Vafa O, Liaw LH, Asch R, Berns MW: Micromanipulation of gametes using laser microbeams. Hum Reprod 1991;6(7):1011–1016
Tadir Y, Neev J, Berns MW: Lasers in micromanipulation of preimplantation embryos and gametes. Semin Reprod Endocrinol 1994;12:169–176
El-Danasouri I, Westphal LM, Neev Y: Assisted fertilization of mouse oocytes using a 308 nm excimer laser microbeam to open the zona pellucida. J Assist Reprod Genet 1993;Suppl:abstr 228
Neev J, Gonzalez A, Licciardi F, Alikani M, Tadir Y, Berns MW, Cohen J: Opening of the mouse zona pellucida by laser without a micromanipulator. Hum Reprod 1993;8(6):939–944
Liow SL, Ng SC, Bongso A, Ratnam SS: Fertilization rates, embryonic development and implantation of laser zona-cut 1- and 2-hole mouse oocytes inseminated at different sperm concentrations. J Assist Reprod Genet 1995;12(3):108 S
Ng SC, Liow SL, Bongso A, Kumar J, Ratnam SS: The use of noncontact microbeam laser for assisted reproduction. J Assist Reprod Genet 1995;12(3):7S
Obruca A, Strohmer H, Sakkas D, Menezo Y, Kogosowski A, Barak Y, Feichtinger W: Use of lasers in assisted fertilization and hatching. Hum Reprod 1994;9(9):1723–1726
Tadir Y, Wright WH, Vafa O, Ord T, Asch R, Berns MW: Micromanipulation of sperm by a laser generated optical trap. Fertil Steril 1989;52(5):870–873
Colon JM, Sarosi P, McGovern PG, Ashkin A, Dziedzic JM, Skurnick J, Weiss G, Bonder EM: Controlled micromanipulation af human sperm in three dimensions with an infrared laser optical trap: Effect of sperm velocity. Fertil Steril 1992;57:695–698
Westphal LM, El-Danasouri I, Shimizu S, Tadir Y: Exposure of human spermatozoa to the cumulus oophorus results in increased relative force as measured by a 760 nm laser optical trap. Hum Reprod 1993;8(7):1083–1086
Schütze K, Clement-Sengewald A, Ashkin A: Zona drilling and sperm insertion with combined laser microbeam and optical tweezers. Fertil Steril 1994;61:783–786
Gordts S, Enginsu E, Bassi S, Pensis M, Campo R, Schütze K, Donnez J: Combined use of an ultraviolet and infrared laser beam in the micromanipulation of mouse embryos. J Assist Reprod Genet 1995;12(3):108S
Liow SL, Ng SC, Vasuthevan S, Tok V, Bongso A, Ratnam SS: Laser zona cutting of mouse oocytes. Theriogenology 1994;41:239
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Clement-sengewald, A., Schütze, K., Ashkin, A. et al. Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. J Assist Reprod Genet 13, 259–265 (1996). https://doi.org/10.1007/BF02065947
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DOI: https://doi.org/10.1007/BF02065947