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Marine Biology

, Volume 111, Issue 1, pp 65–69 | Cite as

Occurrence of tetrodotoxin in the gastropodsRapana rapiformis andR. venosa venosa

  • D. F. Hwang
  • S. C. Lu
  • S. S. Jeng
Article

Abstract

Paralytic toxicity was detected in four of 17 specimens and six of 28 specimens of the gastropodsRapana rapiformis andR. venosa venosa, respectively, collected from Chiching (Kaohsiung City) and Nanfangao (Ilan County), Taiwan, in 1988 and 1989. The highest toxicities, calculated as tetrodotoxin (TTX) content, were 140 and 13 mouse units (MU) g−1 digestive gland inR. rapiformis andR. venosa venosa, respectively. The toxins obtained from each species were purified by ultrafiltration and Bio-Gel P-2 column chromatography. The toxin's specific toxicities (as TTX) were 56 MU mg−1 (R. rapiformis) and 52 MU mg−1 (R. venosa venosa). Results of analyses by thin-layer chromatography, cellulose acetate membrane electrophoresis and high-performance liquid chromatography showed that TTX and anhydrotetrodotoxin were responsible for the toxicity. The alkali hydrolysate of each toxin showed maximum absorption at ca. 274 nm, which is the unique absorption for the C9-base of TTX and its related substances (such as anhydrotetrodotoxin, 4-epitetrodotoxin, etc.). Here, we report for the first time the occurrence of TTX in the gastropodsR. rapiformis andR. venosa venosa.

Keywords

Cellulose Toxicity Electrophoresis Column Chromatography Cellulose Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • D. F. Hwang
    • 1
  • S. C. Lu
    • 1
  • S. S. Jeng
    • 1
  1. 1.Department of Marine Food ScienceNational Taiwan Ocean UniversityKeelungTaiwan, Republic of China

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