Pharmaceutisch Weekblad

, Volume 5, Issue 4, pp 172–176 | Cite as

Capillary gas chromatography on fused silica columns with splitless injection: the determination of phenobarbital in blood plasma

  • J. P. Franke
  • J. Wijsbeek
  • R. A. de Zeeuw
Original Articles


A method is described for the quantitative determination of phenobarbital in blood plasma by means of fused silica capillary columns and splitless sample introduction. Several factors that influence the splitless sample introduction are evaluated. Using a simple column isolation procedure without derivatization prior toGc analysis, recoveries over 80% were found with a precision corresponding with a standard deviation of about 5%. Concentrations ranged from 5–25 mg/l. With a flame ionization detector a detection limit of 0.5 mg/l was observed so that the procedure can also be useful for barbiturates with lower therapeutic plasma levels.


Plasma Level Capillary Column Blood Plasma Quantitative Determination Ionization Detector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • J. P. Franke
    • 1
  • J. Wijsbeek
    • 1
  • R. A. de Zeeuw
    • 1
  1. 1.Department of ToxicologyState University GroningenAW GroningenThe Netherlands

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