Determination of glutathione in biological material by high pressure liquid chromatography
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A rapid and selective determination of reduced glutathione in biological material is described, based on its conjugation with 1-chloro-2,4-dinitrobenzene. The reaction product has aUv absorption maximum at 340 nm and is analysed by reversed phase high pressure liquid chromatography. Linear calibration graphs were obtained in the concentration range between 50μ M and 2 mM glutathione in standard solutions and in biological material (rat liver and bacterial homogenates). The detection limit is about 2μM glutathione when using 20μl injection samples.
KeywordsGlutathione Reversed Phase Biological Material Reduce Glutathione High Pressure Liquid Chromatography
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