Summary
Purified rice dwarf phytoreovirus preparations, after rehydration following drying without freezing (L-drying) and sucrose density gradient centrifugation, sedimented to the same position as untreated controls. Upon storage at 65 °C, virion conformation in L-dried preparations supplemented with 1% sucrose was maintained better than without additives. Moreover, during storage for 6 years at −70 °C, infectivity of L-dried preparations from crude extracts of infected rice plants containing 5% sucrose was higher than controls based on the number of count of infected foci on cell monolayers and transmission to rice seedlings by leafhopper of the vectorNephotettix cincticeps, which had been injected with such extracts.
References
Banno I, Sakane T (1979) Variability of various bacteria drying. IFO Res Comm 9: 34–45
Black LM, Wolcyrz S, Whitcomb RF (1958) A vectorless strain of wound-tumor virus. 7th International Congress for Microbiology, Stockholm, Abstract 14a
Fukumoto F (1996) Long-term persistence of infectivity of the infected crude extracts and leaf tissues of tobacco ringspot virus by L-drying, freeze-drying and desiccation. Ann Phytopathol Soc Japan 62: 49–52
Fukumoto F, Tochihara H (1984) Effect of various additives on the long-term preservation of tobacco ringspot and radish mosaic viruses. Ann Phytopathol Soc Japan 50: 158–165
Fukumoto F, Tochihara H (1985) Effect of various additives on preservation of southern bean mosaic virus. Ann Phytopathol Soc Japan 51: 413–420
Fukumoto F, Tochihara H (1990) Stability of southern bean mosaic and carnation mottle viruses during the preservation by freeze-drying. Ann Phytopathol Soc Japan 56: 47–55
Fukumoto F, Tochihara H (1992) Similarity of the conditions for freeze-drying preservation among three cucumoviruses. Ann Phytopathol Soc Japan 58: 366–372
Fukumoto F, Tochihara H (1996) Comparison between L-drying and freeze-drying methods on the preservation of purified tobacco ringspot virus. Ann Phytopathol Soc Japan 62: 45–48
Grieff D, Rightsel W (1965) An accelerated storage test for predicting the stability of suspensions of measles virus dried by sublimation in vacuo. J Immunol 94: 394–400
Ijima T, Sakane T (1973) A method for preservation of bacteria and bacteriophages by drying in vacuo. Cryobiology 10: 379–385
Kaper JM (1973) Arrangement and identification of simple isometric viruses according to their dominating stabilizing interactions. Virology 55: 299–304
Kimura I (1976) Loss of vector-transmissibility in an isolate of rice dwarf virus. Ann Phytopathol Soc Japan 42: 322–324
Kimura I (1976) Improved purification of rice dwarf virus by the use of polyethylene glycol. Phytopathology 66: 1470–1474
Kimura I (1986) A study of rice dwarf virus in vector cell monolayers by fluorescent antibody focus counting. J Gen Virol 76: 2119–2124
Kimura I, Minobe Y, Omura T (1987) Changes in a nucleic acid and a protein component of rice dwarf virus particles associated with increase of symptom severity. J Gen Virol 68: 3211–3215
Kimura I, Omura T, Minobe Y (1984) Transmission mechanism of rice dwarf virus by the vector leafhopper. Ann Phytopathol Soc Japan 50: 430–431 (Abstract in Japanese)
Mikata K, Yamauchi S, Banno I (1983) Preservation of yeast cultures by drying: variability of 1710 yeasts after drying and storage. IFO Res Comm 11: 25–46
Maoka T, Omura T, Harjosudarmo J, Usugi T, Hibino H, Tsuchizaki T (1993) Loss of vector-transmissibility by maintaining rice ragged stunt virus in rice plants without vector transmission. Ann Phytopathol Soc Japan 59: 185–187
Omura T, Morinaka T, Inoue H, Saito Y (1982) Purification and some properties of rice gall dwarf virus, a new phytoreovirus. Phytopathology 72: 1246–1249
Shikata E (1981) Reoviruses. In: Kurstak E (ed) Handbook of plant virus infection and comparative diagnosis. Elsevier/North-Holland, Amsterdam, pp 423–451
Takahashi Y, Tomiyama M, Hibino H, Omura T (1994) Conserved primary structures in core capsid proteins and reassembly of core particles and outer capsids between rice gall dwarf and rice dwarf phytoreoviruses. J Gen Virol 75: 269–275
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fukumoto, F., Omura, T. & Kimura, I. Effect of protectants in L-drying on the conformation and infectivity of rice dwarf phytoreovirus. Archives of Virology 141, 1579–1585 (1996). https://doi.org/10.1007/BF01718256
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01718256