Skip to main content
SpringerLink
Log in

Secretion of phospholipase A2 induced by interactions of human platelets with monocytes

  • Original Article
  • Published:
Annals of Hematology Aims and scope Submit manuscript

Summary

Phospholipase A2 (PLA2) activity was found and measured in the cell-free supernatants of human mononuclear cells (monocytes and lymphocytes) cultured with platelets for 48 h at 37° C. The relative molecular mass of purified, calcium-dependent PLA2 was 14 kD. The amount of PLA2 in the supernatants correlated positively with the number of monocytes and platelets in the cultures. Electron microscopically, direct cell-to-cell interactions of monocytes and platelets were observed. Cultivation of suspensions of human mononuclear cells with platelets in serum-free medium was found to be an efficient way to produce and purify human secretable PLA2. In the release of secretable PLA2 in peripheral blood, the interactions between platelets and monocytes may play a considerable role.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Batchelor JR (1967) Assays for cytotoxic and haemagglutinating antibodies against histocompatibility antigens. In: Weir DM (ed) Handbook of experimental immunology. Blackwell Scientific, Oxford, p 999

    Google Scholar 

  2. Bing RJ, Dudek R, Kahler J, Narayan KS, Ingram M (1992) Cytokine production from freshly harvested human mononuclear cells attached to plastic beads. Tissue and Cell 24:203–209

    PubMed  Google Scholar 

  3. Boyum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest 21 [Suppl 97]: 9–109

    Google Scholar 

  4. Braquet P, Rola-Pleszczynki M (1987) Platelet-activating factor and cellular immune responses. Immunol Today 8:345–352

    Google Scholar 

  5. Chang J, Gilman SC, Lewis AJ (1986) Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL-1 action. J Immunol 136:1283–1287

    PubMed  Google Scholar 

  6. Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 250:4007–4021

    Google Scholar 

  7. Hoffman GE, Schneider E, Brand K, Kozumplik V, Fateh-Moghadam S (1992) Characterization of a phospholipase A2 in human serum. Eur J Clin Chem Clin Biochem 30:111–117

    PubMed  Google Scholar 

  8. Horsburg CR, Clark RA, Kirkpatrick CH (1987) Lymphokines and platelets promote human monocyte adherence to fibrinogen and fibronectin in vitro. J Leukoc Biol 41:14–24

    PubMed  Google Scholar 

  9. Gearing AJH, Newman W (1993) Circulating adhesion molecules in disease. Immunol Today 14:506–512

    PubMed  Google Scholar 

  10. Kramer RM, Johansen B, Heisson C, Pepinsky RB (1990) Structure and properties of a secretable phospholipase A2 from human platelets. Adv Exp Med Biol 275:35–53

    PubMed  Google Scholar 

  11. Kudo I, Murakami M, Hara S, Inoue K (1993) Mammalian non-pancreatic phospholipase A2. Biochem Biophys Acta 117:217–231

    Google Scholar 

  12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  13. Larsen E, Celi A, Gilbert GE, Furie BC, Erban JK, Bonfarti R, Wagner DD, Furie B (1989) PADGEM protein: a receptor that mediates the infiltration of activated platelets with neutrophils and monocytes. Cell 59:305–312

    PubMed  Google Scholar 

  14. Long MW (1992) Blood cell cytoadhesion molecules. Exp Hematol 20:288–301

    PubMed  Google Scholar 

  15. Mueller HW, Pritzker CR, Kubik A, Deykin D (1993) Characterization of phospholipase A2 secretion from human platelets. Thromb Res 72:519–530

    PubMed  Google Scholar 

  16. Nagy-Vezekenyi K, Szabolcsi M, Szegedi Gy, Sipka S, Dezso B, Szabo G (1980) Wirkung von Lymphokinen auf Hautkarzinome. Z Hautkr 55:1630–1636

    PubMed  Google Scholar 

  17. Nakano T, Arita H (1990) Enhanced expression of group II phopholipase A2 gene in tissues of endotoxin shock rats and its suppression by glucocorticoids. FEBS Lett 261:171–174

    PubMed  Google Scholar 

  18. Pfeilschifter J, Pignat W, Marki F, Wiesenberg J (1989) Release of phospholipase A2 activity from rat vascular smooth muscle cells mediated by cAMP. Eur J Biochem 181:237–242

    PubMed  Google Scholar 

  19. Pfeilschifter J, Pignat W, Vosbek K, Marki F (1989) Interleukin 1 and tumor necrosis factor synergetically stimulate prostaglandin synthesis and phospholipase A2 release from rat renal mesangial cells. Biochem Biophys Res Commun 159:385–394

    PubMed  Google Scholar 

  20. Pruzanski W, Vadas P (1988) Secretory synovial fluid phospholipase A2 and its role in the pathogenesis of inflammation in arthritis. J Rheumatol 15:1601–1603

    PubMed  Google Scholar 

  21. Pruzanski W, Wilmore DW, Suffredini A, Matrich GD, Hoff-mann GD, Browning JL, Stefanksi E, Sternby B, Vadas P (1992) Hyperphospholipasemia A2 in human volunteers challenged with intravenous endotoxin. Inflammation 16:561–570

    PubMed  Google Scholar 

  22. Shibata Y, McCaffrey PG, Miniwasa J, Volkman A, Oghiso Y (1992) Regulation of phospholipase A2 activation and arachidonic acid metabolism in an interleukin-3-dependent macrophage-like cell line. J Leukoc Biol 51:32–38

    PubMed  Google Scholar 

  23. Silverstein RL, Nachman RL (1987) Thrombospondin binds to monocytes-macrophages and mediates platelet-monocyte adhesion. J Clin Invest 79:867–874

    PubMed  Google Scholar 

  24. Sipka S, Laczko J, Szabados S, Fust Gy, Suranyi P, Csipo I, Stenszky V, Nyirkos P, Szegedi Gy (1991) Phagocytosis of autologous platelets by human neutrophil granulocytes. Acta Morphol Hung 39:97–105

    PubMed  Google Scholar 

  25. Sixma JJ (1986) Platelet morphology. In: Holmsen H (ed) Platelet responses and metabolism. CRP Press, Boca Raton, pp 33–61

    Google Scholar 

  26. Stefanski E, Pruzanski W, Sternby B, Vadas P (1986) Purification of a soluble phospholipase A2 from synovial fluid in rheumatoid arthritis. J Biochem 100:1297–1303

    PubMed  Google Scholar 

  27. Vadas P, Hay JB (1980) The release of phospholipase A2 from aggregated platelets and stimulated macrophages of sheep. Life Sci 26:1721–1729

    PubMed  Google Scholar 

  28. Vadas P, Pruzanski W, Stefanski E, Ellies LG, Aubin JE, Sos A, Melcher A (1991) Extracellular phospholipase A2 secretion is a common effector pathway of interleukin-1 and tumor necrosis factor action. Immunol Lett 28:187–194

    PubMed  Google Scholar 

  29. Vadas P, Pruzanski W, Stefanski E, Mustard R, Bohnen J, Fraser I, Farawel V, Bombarder C (1988) Pathogenesis of hypotension in septic shock: correlation of circulating phospholipase A2 levels with circulatory collapse. Crit Care Med 16:1–7

    PubMed  Google Scholar 

  30. Zahler P, Kramer R (1981) Isolation of phospholipase A2 from red cell membrane of sheep. Methods Enzymol 71:680–688

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Third Department of Internal Medicine, University Medical School, 4004, H-Debrecen, Hungary

    S. Sipka, I. Csipo & G. Szegedi

  2. Central Experimental Laboratory, University Medical School, Debrecen, Hungary

    J. Laczko & S. Szabados

  3. Department of Medical Chemistry, University Medical School, Debrecen, Hungary

    P. Gergely

  4. Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

    T. Farkas

  5. EGIS Laboratory, Budapest, Hungary

    L. Bali

  6. Institut Henri Beaufour, Le Plessis Robinson, France

    M. Koltai

Authors
  1. S. Sipka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Farkas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Gergely
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Bali
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Laczko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Szabados
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. Csipo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Koltai
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. G. Szegedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sipka, S., Farkas, T., Gergely, P. et al. Secretion of phospholipase A2 induced by interactions of human platelets with monocytes. Ann Hematol 69, 307–310 (1994). https://doi.org/10.1007/BF01696560

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01696560

Key words

Search

Navigation