Abstract
Oocyte collection was carried out in 53 patients by ultrasonically guided abdominal puncture under local or epidural anesthesia. Follicles were aspirated and flushed two to six times using a syringe with culture medium. A total of 196 oocytes was collected and 84 of those (42.9%) were found in the flushes. Mechanical damage was observed in 5.1% of the oocytes. Cleavage rates in mature oocytes (157) after 48 hr in culture were similar in the aspirate group (56.5%) and in the flush group (54.2%). Ten clinical pregnancies were obtained, corresponding to a pregnancy rate of 18.9%.
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References
Baker ML, Dalrymple GV: Biologic effects of diagnostic ultrasound: A review. Radiology 1978;126:479–483
Matre K, Eik-Nes SH: Biologiske virkninger av ultralyd og mulige skader ved diagnostisk bruk. Tidsskr Nor Laegefore 1983,1497–1501
The Bioeffects Committee of the American Institute of Ultrasound in Medicine: Safety Considerations for Diagnostic Ultrasound, AIUM Publication 316. AIUM, Washington, DC, 1984, pp 1–15
Lenz S: Percutaneous oocyte recovery using ultrasound. Clin Obstet Gynaecol 1985;12:785–798
Carstensen EL, Flynn HG: The potential for transient cavitation with microsecond pulses of ultrasound. UMB 1982;8:L720-L724
Bailey KI, O'Brien WD, Dunn F: Ultrasonically induced morphological damage to mouse ovaries. UMB 1983;9:25–31
Carstensen EL, Child SZ, Lam S, Miller DL, Nyborg WL: Ultrasonic gas-body activation in Drosophilia. UMB 1983;9:473–477
The Bioeffects Committee of The American Institute of Ultrasound in Medicine: Evaluation of Research Reports: Bioeffects Literature Reviews, AIUM Publication 316. AIUM, Washington, DC, 1984, pp 1–35
Veeck LL, Wortharm JWE, Witmyer J, Sandow BA, Acosta AA, Garcia JE, Jones GS, Jones HW: Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 1983;39:594–602
Wood C, McMaster R, Rennie G, Trounson A, Leeton J: Factors influencing pregnancy rates following in vitro fertilization and embryo transfer. Fertil Steril 1985;43:245–250
Edwards RG, Steptoe PC: Current status of in-vitro fertilisation and implantation of human embryos. Lancet 1983;2:1265–1269
Jones HW, Acosta AA, Andrews MC, Garcia JE, Jones GS, Mayer J, McDowell JS, Rosenwaks Z, Sandow BA, Veeck LL, Wilkes CA: Three years of in vitro fertilization at Norfolk. Fertil Steril 1984;42:826–834
Trounson A: In vitro fertilization. Curr Topics Exp Endocrinol 1983;5:43–73
Quinn P, Warnes GM, Kerin JF, Kirby C: Culture factors affecting the success rate of in vitro fertilization and embryo transfer. Ann NY Acad Sci 1985;442:195–204
Acosta AA, Jones GS, Garcia JE, Sandow B, Veeck L, Mantzavinos T: Correlation of human menopausal gonadotropin/human chorionic gonadotropin stimulation and oo-cyte quality in an in vitro fertilization program. Fertil Steril 1984;41:196–201
Oltras CM, Testart J: Factors associated with fractured zonae pellucidae in an in-vitro fertilization programme. Hum Reprod 1986;1:175–180
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Lenz, S., Lindenberg, S., Fehilly, C. et al. Are ultrasonic-guided follicular aspiration and flushing safe for the oocyte?. J Assist Reprod Genet 4, 159–161 (1987). https://doi.org/10.1007/BF01555463
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DOI: https://doi.org/10.1007/BF01555463