Abstract
Extensive research has been conducted in domestic animals, particularly in cattle, in the reproductive technologies of sperm handling, capacitation, and acrosome reaction, superovulation, and embryo handling, sexing, bisection, cryopreservation, and transfer. Because of the economic importance of cattle these technologies have been tested and improved under clinical conditions: The results of employing these procedures are available on tens of thousands of pregnancies and offspring. This information has implications in applying some of the same technologies in human reproduction. The large number of normal progeny produced in cattle after a long prenatal development period, similar to humans, provides some assurance that these technologies, carefully applied, are safe. The basis for these conclusions is documented in the publications cited in this review.
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References
Foote RH: In vitro fertilization in perspective, relative to the science and art of domestic animal reproduction. Appendix, HEW Support of Research Involving Humanin Vitro Fertilization and Embryo Transfer, 1979
Seppälä M, Edwards RG:In vitro fertilization and embryo transfer. Ann NY Acad Sci 1985;442
Hasler JF, McCauley AD, Schermerhorn EC, Foote RH: Superovulatory response of Holstein cows. Theriogenology 1983;19:83–99
Hasler JF, McCauley AD, Lathrop WF, Foote RH: Effect of donor-embryo-recipient interactions on pregnancy rate in a large scale bovine embryo transfer program. Theriogenology 1987;27:139–168
Looney CR: Superovulation in beef females. Proc 5th Ann Conv Am Embryo Transfer Assoc, 1986, pp 16–29
Shea BF, Janzen RE, McDermand DP: Seasonal variation in response to stimulation and related embryo transfer procedures over a nine year period. Theriogenology 1984;21: 186–195
Roberts SJ: Veterinary Obstetrics and Genital Diseases (Theriogenology). Woodstock, VT, 1986, p 110
Adams CE (ed): Mammalian Egg Transfer. Boca Raton, FL, CRC Press, 1982
Armstrong DT, Evans G: Factors influencing success of embryo transfer in sheep and goats. Theriogenology 1983; 19:34–41
Betteridge KJ (ed): Embryo transfer in farm animals. Monogr 16, Can Dept Agr, Ottawa, 1977
Betteridge KJ: An historical look at embryo transfer. J Reprod Fert 1981;62:1–13
Brackett BG, Seidel GE Jr, Seidel SM (eds): New Technologies in Animal Breeding. New York, Academic Press, 1981
Daniel JC Jr (ed): Methods in Mammalian Reproduction. New York, Academic Press, 1978
Foote RH: Embryo transfer and new biotechnology. Proc Vth World Congr Anim Prod 1983;1:59–65
Foote RH, Onuma H: Superovulation, ovum collection, culture and transfer. A review. J Dairy Sci 1970;53:1681–1692
Rinfret AP, Petricciani JC (eds): The Integrity of Frozen Spermatozoa. Washington, DC, Natl Acad Sci, 1978
Rowson LEA, Moor RM, Lawson RAS: Fertility following egg transfer in the cow; Effect of method, medium and synchronization of oestrus. J Reprod Fert 1969;18:517–523
Salisbury GW, VanDemark NL, Lodge JR: Physiology of Reproduction and Artificial Insemination of Cattle. San Francisco, WH Freeman, 1978
Seidel GE Jr: Applications of embryo preservation and transfer.In Animal Reproduction, Beltsville Symposia in Agricultural Research III, HH Hawk (ed). Montclair, NJ, Alanheld, Osmun, 1978
Seidel GE Jr: Superovulation and embryo transfer in cattle. Science 1981;211:351–358
Seidel GE Jr: Mammalian oocytes and preimplantation embryos as methodological components. Biol Reprod 1983; 28:36–49
Brackett BG: A review of bovine fertilization in vitro. Theriogenology 1983;19:1–15
Mapletoft RJ: Embryo transfer technology for the enhancement of animal reproduction. Biotechnology 1984;2:149–160
Sreenan JM: Embryo transfer procedure and its use as a research technique. Vet Rec 1983;112:494–500
Elsden RP, Nelson LD, Seidel GE Jr: Superovulating cows with follicle stimulating hormone and pregnant mare's serum gonadotrophin. Theriogenology 1978;9:17–26
Gordon I, Williams G, Edwards J: The use of serum gonadotrophin (P.M.S.) in the induction of twin-pregnancy in the cow. J Agr Sci (Cambr) 1962;59:143–198
Bellows RA, Short RE: Superovulation and multiple births in beef cattle. J Anim Sci (Suppl 1) 1972;34:67–79
Gengenbach DR, Butendieck N, Riek PM, Scipioni RL, Oltenacu EB, Foote RH: Controlled superovulation in dairy heifers using prostaglandin F2 alpha and pregnant mare serum gonadotropin. J Anim Sci 1978;46:1293–1299
Anderson GB: Methods for producing twins in cattle. Theriogenology 1978;9:3–26
Murphy BD, Mapletoft RJ, Manns J, Humphrey WD: Variability in gonadotrophin preparations as a factor in the superovulatory response. Theriogenology 1984;21:117–125
Donaldson LE, Ward DN, Glenn SD: Use of porcine follicle stimulating hormone after Chromatographic purification in superovulation of cattle. Theriogenology 1986;25:747–757
Lauria A, Oliva O, Genazani AR, Genomesi F, Gotti S, Barbetti M: Improved method to induce superovulation in cattle using human menopausal gonadotropin. Theriogenology 1982;18:357–364
Bindon BM, Piper LR, Cahill LP, Driancourt MA, O'Shea T: Genetic and hormonal factors affecting superovulation. Theriogenology 1986;25:53–70.
Moor RM, Osborn JC, Crosby IM: Gonadotrophin-induced abnormalities in sheep oocytes after superovulation. J Reprod Fert 1985;74:167–172
Spicer LJ, Echternkamp SE: Ovarian follicular growth, function and turnover in cattle: A review. J Anim Sci 1986;62:428–451
Fortune JE, Hansel W: Concentrations of steroids and gonadotropins in follicular fluid from normal heifers and heifers primed for superovulation. Biol Reprod 1985;32:1069–1079
Hyttel P, Callesen H, Greve T: Ultrastructural features of preovulatory oocyte maturation in superovulated cattle. J Reprod Fertil 1986;76:645–656
Callesen H, Greve T, Hyttel P: Preovulatory endocrinology and oocyte maturation in superovulated cattle. Theriogenology 1986;25:71–86
Bowen RA, Elsden RP, Seidel GE Jr: Embryo transfer for cows with reproductive problems. J Am Vet Med Assoc 1978;172:1303–1307
Erickson BH: Development and senescence of the postnatal bovine ovary. J Anim Sci 1966;25:800–805
Foote RH: The gametogenic function of the aging ovary in the mammal. In Aging Gametes, RJ Blandau (ed). Basel, S Karger, 1975, pp 179–200
Maurer RR, Foote RH: Maternal aging and embryonic mortality in the rabbit. I. Repeated superovulation, embryo culture and transfer. J Reprod Fert 1971;25:329–341
Maurer RR, Hunt WL, Van Vleck LD, Foote RH: Development potential of superovulated rabbit ova. J Reprod Fert 1968;15:171–175
Douglas RJ, Burns PJ, Hershman L: Physiological and commercial parameters for producing progeny from subfertile mares by embryo transfer. Equine Vet J (Suppl 3) 1985;3:111–114
Ball BA, Little TV, Hillman RB, Woods GL: Pregnancy rates at days 2 and 14 and estimated embryonic loss rates prior to day 14 in normal and subfertile mares. Theriogenology 1986;26:611–619
Donaldson LE: Embryo production in superovulated cows; Transferrable embryos correlated with total embryos. Theriogenology 1984;21:517–524
Ginther OJ: Embryonic loss in mares: Incidence, time of occurrence and hormonal involvement. Theriogenology 1985;23:77–89
Foote RH: Semen quality from the bull to the freezer: An assessment. Theriogenology 1975;3:219–235
Elsden RP, Hasler JF, Seidel GE Jr: Non-surgical recovery of bovine eggs. Theriogenology 1976;6:523–532
Trounson AO, Rowson LEA, Willadsen SM: Nonsurgical transfer of bovine embryos. Vet Rec 1978;102:74–75
Imel KJ, Squires EL, Elsden RP, Shideler RK: Collection and transfer of equine embryos. J Am Vet Assoc 1981;79: 987–991
Squires EL, Garcia RH, Ginther OJ: Factors affecting success of equine embryo transfer. Equine Vet J (Suppl 3) 1985;3:92–95
Vogelsang SG, Bondioli KR, Massey JM: Commercial application of equine embryo transfer. Equine Vet J (Suppl 3) 1985;3:89–91
Woods GL, Ginther OJ: Recent studies relating to the collection of multiple embryos in mares. Theriogenology 1983;19:101–108
Tervit HR, Goold PG, McKenzie RD, Clarkson DJ: Techniques and success of embryo transfers in Angora goats. NZ Vet J 1983;31:67–70.
Foote RH: Superovulation practices and related research. Proc 5th Annu Conv Am Embryo Transfer Assoc, 1986, pp 2–12
Schilling E, Smidt D, Sacher D, Petac D, Kaschab E: Diagnosis of the viability of early bovine embryos by fluorescence microscopy. Ann Biol Anim Biochem Biophys 1979;19:1625–1629
Shea BF: Evaluating the bovine embryo. Theriogenology 1981;15:31–42
Greve T, Callesen H, Hyttel P: Endocrine profiles and egg quality in the superovulated cow. Nord Vet Med 1983;35:408–421
Hoppe RW, Bavister BD: Evaluation of the fluorescein diacetate (FDA) vital dye viability test with hamster and bovine embryos. Anim Reprod Sci 1983;6:323–335
Lindner GM, Wright RW: Bovine embryo morphology and evaluation. Theriogenology 1983;20:407–416
Masslip A, Zwijsen W, Mulnard J: Cinematographic analysis of the cleavage of the cow egg from 2-cell to 16-cell stage. Arch Biol (Brussels) 1983;94:99–106
Takeda T, Hasler JF: Effect of plastic disposable syringes on development of mouse embryos in culture. Theriogenology 1986;25:205
Quinn P, Warnes GM, Kerin JF, Kirby C: Culture factors in relation to the success of human in vitro fertilization and embryo transfer. Fertil Steril 1984;41:202–209
Ackerman SB, Stokes GL, Swanson RJ, Taylor SP Fenwick L: Toxicity testing for human in vitro fertilization programs. J Vitro Fert Embryo Transfer 1985;2:132–137
Wright RW Jr, Bondioli KR: Aspects of in vitro fertilization and embryo culture in domestic animals J Anim Sci 1981;53:702–729
Suzuki T, Shimohira I: Cultivation in vitro of bovine embryos in a medium supplemented with bovine serum collected 7–8 days after superovulation. Jap J Anim Reprod 1985;31:l-4
Lindner GM, Ellis DE: Refrigeration of bovine embryos. Theriogenology 1985;23:202
Seidel GE Jr: Applications of embryo transfer and related technologies to cattle. J Dairy Sci 1984;67:2786–2796
Kuzan FB, Wright RW: Observations on the development of bovine morula on various cellular and monocellular substrates. J Anim Sci 1982;54:811–816
Voelkel SA, Amborski GE, Hill KG, Godke RA: Use of a uterine-cell monolayer culture system for micromanipulated bovine embryos. Theriogenology 1985;24:271–282
Whittingham DG, Lyon MF, Glenister PH: Reestablishment of breeding stocks of mutant and inbred strains of mice from embryos stored at −196°C for prolonged periods. Genet Res (Cambr) 1977;30:287–299
Polge C: The freezing of mammalian embryos; Perspectives and possibilities.In The Freezing of Mammalian Embryos, Ciba Foundation Symposium 52. Amsterdam, Elsevier-Excerpta Medica, 1977
Lehn-Jensen H: Deep freezing of cow embryos, a review. Nord Vet Med 1981;32:523–532
Leibo SP: A one-step method for direct nonsurgical transfer of frozen-thawed bovine embryos. Theriogenology 1984;21:767–790
Farrand GD, Elsden RP, Seidel GE Jr: Effect of slow cooling end point temperature on survival of frozen bovine embryos. J Anim Sci 1985;61:460–465
Lehn-Jensen H, Willadsen SM: Deep-freezing of cow “half” and “quarter” embryos. Theriogenology 1983;19:49–54
Leibo SP: Cryobiology: Preservation of mammalian embryos.In Genetic Engineering of Animals, JW Evans, A Hollander (eds). New York, Plenum Press, 1986, pp 251–272
Czlonkowska M, Boyle MS, Allen WR: Deep freezing of horse embryos. J Reprod Fert 1985;75:485–490
Slade NP, Takeda T, Squires EL, Elsden RP, Seidel GE Jr: A new procedure for the cryopreservation of equine embryos. Theriogenology 1985;24:45–48
Hare WCD: Diseases transmissable by semen and embryo transfer techniques. Office Inter des Epizost, Paris, 1985
Lehn-Jensen H, Rall WF: Cryomicroscopic observation of cattle embryos during freezing and thawing. Theriogenology 1983;19:263–278
Hyttel P, Lehn-Jensen H, Greve T: Ultrastructure of bovine embryos frozen and thawed by a two-step freezing method. Acta Anat 1986;125:27–31
Wright JM; Commercial freezing of bovine embryos in straws. Theriogenology 1985;23:17–29
Merry CA, Bondioli KR, Allen RL, Wright RW Jr: One-step sucrose dilution of frozen-thawed sheep embryos. Theriogenology 1984;22:433–441
Bowen MJ, Salsbury JM, Bowen JM, Kramer DC: Non-surgical embryo auto-transfer in the mare. Equine Vet J Suppl 1985;3:100–102
Iuliano MF, Squires EL, Cook VM: Effect of age of equine embryos and method of transfer on pregnancy rate. J Anim Sci 1985;60:258–263
Wilmut I, Sales DI, Ashworth CJ: The influence of variation in embryo stage and maternal hormone profiles on embryo survival of farm animals. Theriogenology 1985;23:107–119
Ginther OJ: Effect of reproductive status of twinning and on side of ovulation and embryo attachment in mares. Theriogenology 1983;20:383–395
Pope WF, First NL: Factors affecting the survival of pig embryos. Theriogenology 1985;23:91–105
Northey DL, Barnes FL, Eyestone WH, First NL: Relationship of serum progesterone, luteinizing hormone and the incidence of pregnancy in bovine embryo transfer recipients. Theriogenology 1985;23:214
Markette KL, Seidel GE Jr, Elsden RP: Estimation of embryonic losses in bovine embryo transfer recipients from progesterone profiles and returns to estrus. Theriogenology 1985;23:45–62.
Allen WR, Kydd J, Boyle MS, Antczak DF: Betweenspecies transfer of horse and donkey embryos: A valuable research tool. Equine Vet J (Suppl 3) 1985;23:53–62
King KK, Seidel GE Jr, Elsden RP: Bovine embryo transfer pregnancies. I. Abortion rates and characteristics of calves. J Anim Sci 1985;61:747–757
Biggers JD; The potential use of artificially produced monocygotic twins for comparative experiments. Theriogenology 1986;26:l-25
Hare WCD, Betteridge KJ: Relationship of embryo sexing to other methods of prenatal sex determination in farm animals: a review. Theriogenology 1978;9:27–43
King WA: Sexing embryos by cytological methods. Theriogenology 1984;21:7–17
Anderson GB: Identification of sex in mammalian embryos.In Genetic Engineering in Animals, JW Evans, A Hollaender (eds). New York, Plenum Press, 1986, pp 243–250
Amann RP, Seidel GE Jr (eds): Prospects for Sexing Mammalian Sperm. Boulder, CO, Colo Assoc Univ Press, 1982
Foote RH: Normal development of fetuses resulting from Holstein semen processed for sex separation. Theriogenology 1985;24:197–202
Garner DL, Gledhill BL, Pinkel D, Lake S, Stephenson D, VanDilla M, Johnson LA: Quantification of the X- and Y-chromosome-bearing spermatozoa of domestic animals by flow cytometry. Biol Reprod 1983;28:312
Zavos PM: Preconception sex determination via intravaginal administration of H-Y antisera in rabbits. Theriogenology 1983;20:235–240
Willadsen SM, Lehn-Jensen H, Fehilly CD, Newcomb R: The production of monozygotic twins of preselected parentage by micromanipulation of nonsurgically collected cow embryos. Theriogenology 1981;15:23–30
Ozil JP: Production of identical twins by bisection of blastocysts in the cow. J Reprod Fert 1983;69:463–468
Ozil JP, Heyman Y, Renard JP: Production of monozygotic twins by micromanipulation and cervical transfer in the cow. Vet Rec 1982;110:126–127
Williams TJ, Seidel GE Jr: Methodology and equipment for microsurgery with mammalian ova. Proc Workshop IXth Annu Mtg Int Embryo Transfer Soc, 1983, pp 33–52
Baker RD, Shea BF: Commercial splitting of bovine embryos. Theriogenology 1985;23:3–12
Tsunoda Y, Tokunaga T, Sugie T, Katsumata M: Production of monozygotic twins following the transfer of bisected embryos in the goat. Theriogenology 1985;24:337–344
Willadsen SM, Godke RA: A simple procedure for the production of identical sheep twins. Vet Rec 1984;114:240–243
Critser ES, Ball GD, O'Brien MJ, First NL: Use of a fluorescent stain for visualization of nuclear material in living gametes and early embryos. Biol Reprod (Suppl 1) 1983;28:140
Wall RJ, Pursell VG, Hammer RF, Brinster RL: Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei. Biol Reprod 1985;32:645–652
Hammer RE, Pursel VG, Rexroad CE Jr, Wall RJ, Bolt DJ, Ebert KM, Palmiter RD, Brinster RL: Production of transgenic rabbits, sheep and pigs by microinjection. Nature 1985;315:680–683
Marken CL: Fertilization of mammalian eggs by sperm injection. J Exp Zool 1983;228:195–201
Willadsen SM: Nuclear transplantation in sheep embryos. Nature 1986;320:63–65
Katz DF, Overstreet JW: Sperm motility assessment by video-micrography. Fertil Steril 1981;35:188–193
Ericsson RJ: Isolation and storage of progressively motile human sperm. Andrologia 1977;9:111–114
Wall RJ, Jerrard DA, Parrish JJ, Foote RH: Optimization of procedures for separation of motile and nonmotile bull and rabbit spermatozoa with bovine serum albumin gradients. Gamete Res 1984;10:165–175
White LM, Beal WE, Bame JH, Saacke RG, Marshall CE: Characteristics of bovine spermatozoa after migration through a bovine serum albumin gradient. J Anim Sci 1984;59:454–459
Lorton SP, Kummerfeld HL, Foote RH: Polyacrylamide as a substitute for cervical mucus in sperm migration tests. Fertil Steril 1981;35:222–227
Rogers BJ: The usefulness of the sperm penetration assay in predicting in vitro fertilization (IVF) success. J Vitro Fert Embryo Transfer 1986;3:209–211
Parrish JJ, Foote RH: Fertility differences among male rabbits determined by heterospermic insemination of fluorochrome labeled spermatozoa. Biol Reprod 1985;32:940–949
Parrish JJ, Susko-Parrish JL, First NL: In vitro fertilization of bovine oocytes using heparin treated and swimup separated frozen thawed bovine semen is repeatable and results in high frequencies of fertilization. Theriogenology 1985;23:216 (abstr)
Langlais J, Roberts KD: A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa. Gamete Res 1985;12:183–224
Lenz RW, Ball GD, Lohse JK, First NL, Ax RL: Chondroitin sulfate facilitates an acrosome reaction in bovine spermatozoa as evidenced by light microscopy, electron microscopy and in vitro fertilization. Biol Reprod 1983;28:683–690
Iritani A, Kasai M, Niwa K, Song HB: Fertilizationin vitro of cattle follicular oocytes with ejaculated spermatozoa capacitated in a chemically defined medium. J Reprod Fertil 1984;70:487–492
Takahashi Y, Hanada A: Penetration of zona-free hamster eggs in vitro by ejaculated bull spermatozoa after treatment with ionophore A23187. Jap J Anim Reprod 1984;30:30–38
Graham JK, Foote RH, Parrish JJ: Effect of dilauroylphosphatidlycholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs. Biol Reprod 1986;34:413–424
Ax RL, Dickson K, Lenz RW: Induction of acrosome reactions by chondroitin sulfates in vitro corresponds to nonreturn rates of dairy bulls. J Dairy Sci 1985;68:387–390
Graham JK, Foote RH: Dilauroylphosphatidylcholine liposome effects on the acrosome reaction and in vitro penetration of zona-free hamster eggs by bull sperm. I. A fertility assay for fresh semen. Gamete Res 1987;16:133–146
Graham JK, Foote RH: Dilauroylphosphatidylcholine liposome effects on the acrosome reaction and in vitro penetration of zona-free hamster eggs by bull sperm. II. A fertility assay for frozen-thawed semen. Gamete Res 1978; 16:147–158
Yanagimachi R, Yanagimachi H, Rogers BJ: The use of zona-free animal ova as a test system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 1976;15:471–476
Graham JK: Changes Induced in Spermatozoa by Liposomes Which Promote Sperm Cryopreservation, the Acrosome Reaction and Sperm Penetration into Zona-Free Hamster Eggs, PhD thesis. Ithaca, NY, Cornell University, 1985
Davis AP, Graham JK, Foote RH: In vitro heterospermic insemination of zona-free hamster eggs by fluorochrome-labeled bull sperm: A fertility test. J Dairy Sci (Suppl 1) 1986;69:147 (abstr)
Blazak WF, Overstreet JW, Katz DF, Hanson FW: A competitive in vitro assay of human sperm fertilizing ability utilizing contrasting fluorescent sperm markers. J Androl 1982;3:165–171
Rodriguez H, Ohanian C, Bustos-Obregon E: Nuclear chromatin decondensation of spermatozoa in vitro: A method for evaluating the fertilizing ability of ovine semen. Int J Androl 1985;8:147–158
Graham JK, Foote RH: In vitro fertilization of zona-free ova by liposome treated bovine, equine, ovine and porcine sperm. Biol Reprod (Suppl 1) 1985;32:82 (abstr)
Pitra C, Schafer W, Jewgenow K: Quantitative determination of fertilizing-capacity of deep-frozen stallion sperm by means of hamster oocyte penetration test. Monata Vet 1985;40:235–237
Ball GB, Liebfried ML, Ax RL, First NL: Maturation and fertilization of bovine oocytes in vitro. J Dairy Sci 1984;67:2775–2785
Bousquet D, Goff AK, King WA, Greve T, Betteridge KJ: Fertilization of bovine oocytes in vitro: Analysis of some factors affecting the fertilization rates. 10th Int Congr Anim Reprod AI 1983;III:386 (abstr)
Liebfried-Rutledge ML, Critser ES, First NL: Fertilization potential of follicular oocytes classified by stage of cycle and size of follicle. Theriogenology 1985;23:753–759
Maruska DV, Leibfried ML, First NL: Role of calcium and the calcium-calmodulin complex in resumption of meiosis, cumulus expansion, viability and hyaluronidase sensitivity of bovine cumulus-oocyte complexes. Biol Reprod 1984;31:1–6
Ball GB, Liebfried ML, Lenz RW, Ax RL, Bavister BD, First NL: Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biol Reprod 1983;28:717–725
Critser ES, Leibfried ML, First NL: The effect of semen extension, cAMP and caffeine onin vitro fertilization of bovine oocytes. Theriogenology 1984;21:625–631
Brackett BF, Bousquet D, Boice ML, Donawick WJ, Evans JF: Normal development following in vitro fertilization in the cow. Biol Reprod 1982;27:147–158
Lambert RD, Sirard MA, Bernard C, Beland R, Rioux JE, Leclerc P, Menard DP, Bedoya M: In vitro fertilization of bovine oocytes matured in vivo and collected at laparoscopy. Theriogenology 1986;25:117–134
Sirard MA, Lambert RD: In vitro fertilization of bovine follicular oocytes obtained by laparoscopy. Biol Reprod 1985;33:487–494
Sirard MA, Lambert RD, Menard DP, Bedoya M: Pregnancies after in-vitro fertilization of cow follicular oocytes, their incubation in rabbit oviduct and their transfer to cow uterus. J Reprod Fert 1985;75:551–556
Greve T, Bousquet D, King WA, Betteridge KJ: In vitro fertilization and cleavage of in vitro matured bovine oocytes. Theriogenology 1984;22:151–165
Grimes RW, Ireland JJ: Relationship of macroscopic appearance of the surface of bovine ovarian follicles, concentration of steroids in follicular fluid, and maturation of oocytes in vitro. Biol Reprod 1986;35:725–732
Moor RM, Trounson AD: Hormonal and follicular factors affecting maturation of sheep oocytesin vitro and their subsequent developmental capacity. J Reprod Fert 1977;49:101–109
Eng LA, Kornegay ET, Huntington J, Wellman T: Effects of incubation temperature and bicarbonate on maturation of pig oocytes in vitro. J Reprod Fert 1986;76:657–662
Motlik J, Fulka J: Factors affecting meiotic competence in pig oocytes. Theriogenology 1986;25:87–96
Katsa L, Smorag Z: The influence of culture temperature on in vitro maturation of bovine oocytes. Anim Reprod Sci 1985;9:205–212
Moor RM, Crosby IM: Temperature-induced abnormalities in sheep oocytes during maturation. J Reprod Fert 1985;75: 467–473
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Foote, R.H. In vitro fertilization and embryo transfer in domestic animals: Applications in animals and implications for humans. J Assist Reprod Genet 4, 73–88 (1987). https://doi.org/10.1007/BF01555444
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DOI: https://doi.org/10.1007/BF01555444