Abstract
The development of mouse embryos from two cells to blastocysts in Whittingham's T6 medium containing the growth factor insulin or the serum replacement NuSerum was studied and compared with development in vivo. The rate of embryo development to blastocysts was increased by the addition of Nu-Serum to T6 and there was a significant increase in the implantation rate of embryos grown in T6 + Nu-Serum compared with T6 alone or with insulin. However, the rate of embryo development in vitro was retarded compared with that in vivo and the number of normal fetuses following transfer to recipients of blastocysts grown in T6 + Nu-Serum was not different from the number for those grown in T6 alone or with insulin. The addition of neither insulin nor Nu-Serum to T6 optimizes embryo development or viability.
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References
Trounson A: In vitro fertilization and embryos preservation.In In Vitro Fertilization and Embryo Transfer, A Trounson, C Wood (eds). Edinburgh, Churchill Livingstone, 1984, pp 111–130
Menezo Y, Testart J, Perrone D: Serum is not necessary for human in vitro fertilization, early embryo culture and transfer. Fertil Steril 1984;42:750–755
Quinn P, Kerin JF, Warnes GM: Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985;44:493–498
Caro CM, Trounson A: Successful fertilization, embryo development, and pregnancy in human in vitro fertilization (IVF) using a chemically defined culture medium containing no protein. J Vitro Fert Embryo Transfer 1986;3:215–217
Cholewa JA, Whitten WK: Development of 2-cell mouse embryos in vitro. III. The effect of fixed nitrogen source. J Exp Zool 1965;158:69–78
Caro CM, Trounson A: The effect of protein on preimplantation mouse embryo development in vitro. J Vitro Fert Embryo Transfer 1984;1:183–187
Gardner HG, Kaye PL: Effects of insulin on preimplantation mouse embryos. In Proceedings of the Australian Society of Reproductive Biology, 1984, Abstr 107
Hayashi I, Sato GH: Replacement of serum by hormones permits growth of cells in a defined medium. Nature 1976;259:132–134
Harlow GM, Quinn P: Development of preimplantation mouse embryos in vivo and in vitro. Aust J Biol Sci 1982;35:187–193
Gianaroli L, Seracchioli R, Ferraretti AP, Trounson A, Flamigni C, Bovicelli L: The successful use of human amniotic fluid for mouse embryo culture and human in vitro fertilization, embryo culture and transfer. Fertil Steril 1986;46:907–913
Shirley B, Wortham JWE, Witmyer J, Condon-Mahony M, Fort G: Effects of human serum and plasma on development of mouse embryos in culture medium. Fertil Steril 1985;43:129–134
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Caro, C.M., Trounson, A. & Kirby, C. Effect of growth factors in culture medium on the rate of mouse embryo development and viability in vitro. J Assist Reprod Genet 4, 265–268 (1987). https://doi.org/10.1007/BF01555201
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DOI: https://doi.org/10.1007/BF01555201