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Amino Acids

, Volume 12, Issue 2, pp 139–144 | Cite as

Identification of a specific protein in the mitochondrial fraction of the rat heart whose phosphorylation is inhibited by taurine

  • J. B. Lombardini
Full Papers

Summary

It was previously reported that the mitochondrial fraction of the rat heart contained a specific protein with a molecular weight of approximately ∼44kDa whose phosphorylation was inhibited by taurine (Lombardini,1994a). Isolation of the ∼44kDa phosphoprotein on a 1-dimensional polyacrylamide gel using traditional glycine buffers followed by re-electrophoresing the cut out proportion of the gel which corresponds to the ∼44kDa protein on a tricine-buffered gel resulted in sufficient pure protein for sequence analysis. The results indicate that the ∼44kDa phosphoprotein is pyruvate dehydrogenase.

Keywords

Amino acids Taurine Pyruvate dehydrogenase Mitochondria Rat heart Phosphorylation 

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • J. B. Lombardini
    • 1
  1. 1.Departments of Pharmacology and Ophthalmology and Visual SciencesTexas Tech University Health Sciences CenterLubbockUSA

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