Amino Acids

, Volume 13, Issue 2, pp 183–188 | Cite as

Incorporation of nitrate nitrogen in rice seedlings transferred to anaerobic conditions

  • R. Reggiani
  • F. Bertini
  • M. Mattana
Short Communication


Incorporation of15NO3- into amino acids was studied in 3-day-old aerobic rice seedlings (with coleoptile and root) subjected for 24h to anaerobic conditions. The incorporation of15N into glutamate, glutamine and alanine accounted for 89% and 84% of total incorporation in coleoptile and root, respectively. These findings indicate that, after the primary incorporation of15N into glutamate and glutamine, the main fate of nitrate nitrogen in rice seedlings subjected to anoxia is alanine.


Amino acids Anoxia Coleoptile Nitrate nitrogen Rice Root 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Fan TWM (1994) Nitrate metabolism maintains energy production in anaerobic rice coleoptiles. Proceedings of the Fifth International Symposium on Genetics and Molecular Biology of Plant Nutrition, Davis, CA, pp 71–72Google Scholar
  2. Ferrari TE, Varner JE (1971) Intact tissue assay for nitrate reductase in barley aleurone layers. Plant Physiol 47: 790–794Google Scholar
  3. Ferrari TE, Yoder OC, Filner P (1973) Anaerobic nitrite production by plant cells and tissues. Evidence for two nitrate pools. Plant Physiol 51: 423–431Google Scholar
  4. Gray VM, Cresswell CF (1984) Nitrite utilization by excisedZea mays L. roots under anaerobic conditions. Plant Sci Lett 33: 31–38Google Scholar
  5. Jarrett HW, Cooksy KD, Ellis B, Anderson JM (1986) The separation of ophthalaldehyde derivatives of amino acids by reverse-phase chromatography on octylsilica columns. Anal Biochem 153: 189–198PubMedGoogle Scholar
  6. Jaworski EG (1971) Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun 43: 1274–1279PubMedGoogle Scholar
  7. Kemp K, Small JGC (1993) Nitrate and nitrate reductase inErythrina caffra seeds: enhancement of induction by anoxia and possible role in germination. Planta 189: 298–300Google Scholar
  8. Lee RB (1978) Inorganic nitrogen metabolism in barley roots under poorly aerated conditions. J Exp Bot 29: 693–708Google Scholar
  9. Malavolta E (1954) Study on the nitrogenous nutrition of rice. Plant Physiol 29: 98–99Google Scholar
  10. Mattana M, Bertani A, Reggiani R (1994a) Expression of glutamine synthetase during the anaerobic germination ofOryza sativa L. Planta 195: 147–149Google Scholar
  11. Mattana M, Coraggio I, Bertani A, Reggiani R (1994b) Expression of the enzymes of nitrate reduction during the anaerobic germination of rice. Plant Physiol 106: 1605–1608PubMedGoogle Scholar
  12. Mattana M, Coraggio I, Brambilla I, Bertani A, Reggiani R (1996) Nitrate assimilation during the anaerobic germination of rice: expression of ferredoxin-dependent glutamate synthase. Planta 199: 74–78Google Scholar
  13. Mawhinney TP, Robinett RSR, Atalay A, Madson MA (1986) Analysis of amino acids as their tert.-butyldimethylsilyl derivatives by gas-liquid chromatography and mass spectrometry. J Chomatogr 358: 231–242Google Scholar
  14. Opik H (1973) Effect of anaerobiosis on respiration rate, cytochrome oxidase activity and mitochondrial structures in coleoptile of rice (Oryza sativa L.). L Cell Sci 12: 725–739Google Scholar
  15. Patterson BW, Carraro F, Wolfe RR (1993) Measurement of 15N enrichment in multiple amino acids and urea in a single analysis by gas chromatography/mass spectrometry. Biol Mass Spectrom 22: 518–523PubMedGoogle Scholar
  16. Reggiani R, Bertini F, Mattana M (1995) Incorporation of nitrate nitrogen into amino acids during the anaerobic germination of rice. Amino Acids 9: 385–390Google Scholar
  17. Reggiani R, Brambilla I, Bertani A (1985) Effect of exogenous nitrate on anaerobic metabolism in excised rice roots. 1. Nitrate reduction and pyridine nucleotides pools. J Exp Bot 36: 1193–1199Google Scholar
  18. Reggiani R, Cantù CA, Brambilla I, Bertani A (1988) Accumulation and interconversion of amino acids in rice roots under anoxia. Plant Cell Physiol 29: 981–987Google Scholar
  19. Reggiani R, Mattana M, Aurisano N, Bertani A (1993) Utilization of seed storage nitrate during the anaerobic germination of rice. Plant Cell Physiol 34: 379–383Google Scholar
  20. Robinson SA, Slade AP, Fox GG, Phillips R, Ratcliffe RG, Stewart GR (1991) The role of glutamate dehydrogenase in plant nitrogen metabolism. Plant Physiol 95: 509–516Google Scholar
  21. Singh BK, Matthews BF (1994) Molecular regulation of amino acid biosynthesis in plants. Amino Acids 7:165–174Google Scholar
  22. Yamasaki T, Seino K (1965) Use of nitrate fertilizers for the cultivation of paddy rice (part 1). About the physiological character of rice seedlings supplied with nitrate as the source of nitrogen. J Sci Soil Manure Japan 36: 153–158Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • R. Reggiani
    • 1
  • F. Bertini
    • 2
  • M. Mattana
    • 1
  1. 1.Istituto Biosintesi VegetaliC.N.R.MilanoItaly
  2. 2.Istituto Chimica MacromolecoleC.N.R.MilanoItaly

Personalised recommendations