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Origin ofd-serine present in urine of mutant mice lackingd-amino-acid oxidase activity

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Summary

Urine of ddY/DAO mice lackingd-amino-acid oxidase contained 5.7 times more serine than that of normal ddY/DAO+ mice. Most of the serine wasd-isomer. The origin of thisd-serine was examined. Oral administration of 0.02% amoxicillin and 0.004% minocycline to the ddY/ DAO- mice for 7 days did not reduce the urinaryd-serine, indicating that thed-serine was not of intestinal bacterial origin. When the mouse diet was changed to one with different compositions, the urinaryd-serine was considerably reduced. Furthermore, starvation of the ddY/DAO- mice for 24 hours reduced the urinaryd-serine to 33% of the original level. These results indicate that most of the urinaryd-serine comes from the diet. However, the urine of the starved ddY/DAO- mice still contained 4.6 times mored-serine than that of the ddY/DAO+ mice, suggesting a part of the D-serine have an endogenous origin.

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Authors and Affiliations

  1. Department of Oral Surgery, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan

    S. Asakura

  2. Department of Microbiology, Dokkyo University School of Medicine, Mibu, 321-02, Tochigi, Japan

    R. Konno

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  1. S. Asakura
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  2. R. Konno
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Asakura, S., Konno, R. Origin ofd-serine present in urine of mutant mice lackingd-amino-acid oxidase activity. Amino Acids 12, 213–223 (1997). https://doi.org/10.1007/BF01373002

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  • DOI: https://doi.org/10.1007/BF01373002

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