Summary
High performance liquid chromatography was used to analyze the amino acid composition of cells. A total of 17 amino acids was analyzed. This method was used to compare the amino acid compositions of the following combinations: primary culture and established cells, normal and transformed cells, mammalian and bacterial cells, andEscherichia coli andStaphylococcus aureus. The amino acid compositions of mammalian cells were similar, but the amino acid compositions ofEscherichia coli andStaphylococcus aureus differed not only from mammalian cells, but also from each other. It was concluded that amino acid composition is almost independent of cell establishment and cell transformation, and that the amino acid compositions of mammalian and bacterial cells differ. Thus, it is likely that changes in amino acid composition due to cell transformation or species differences between mammalian cells are negligible compared with the differences between mammalian and bacterial cells, which are more distantly related.
Similar content being viewed by others
References
Akimoto K, Ikeda M, Sorimachi K (1997) Inhibitory effect of heparin on collagen fiber formation in hepatic cells in culture. Cell Struct Funct (in press)
Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using precolumn derivatization. J Chromatogr 336: 93–104
Dawe CJ, Whan-Peng J, Morgan WD (1968) Culture of a cell line (NCLP-6E) derived from a hepatocarcinoma induced in Macaca mulatta by n-nitrosodiethylamine. J Natl Cancer Ist 40: 1167–1193
Hasegawa K, Watanabe K, Koga M (1982) Induction of mitosis in primary cultures of adult rat hepatocytes under serum-free conditions. Biochem Biophys Res Commun 104: 259–265
Ikeda M, Sorimachi K, Akimoto K, Okazaki M, Sunagawa M, Niwa A (1995) Analysis of hydroxyproline in urine by high-performance liquid chromatography after dabsylchloride derivatization. Amino Acids 8: 401–407
Ikeda M, Sorimachi K, Akimoto K, Yasumura Y (1991) Simultaneous analysis of 19 amino acids including hydroxyproline and hydroxylysine by high performance liquid chromatography with ODS-column. Dokkyo J Med Sciences 18: 87–93
Ikeda M, Sorimachi K, Akimoto K, Yasumura Y (1993) Reversed-phase high-performance liquid chromatographic analysis of hydroxyproline and proline from collagen by derivatization with dabsyl chloride. J Chromatogr 621: 133–138
Kakuya T, Yamada T, Yokokawa M, Ueda T (1983) Establishment of cell strains from human urothelial carcinoma and their morphological characterization. In Vitro 19: 591–599
Katsuta H, Takaoka T (1968) Cytobiological transformation of normal rat liver cells by treatment with 4-dimethylaminoazobenzene after Nagisa Culture. In: Katsuta H (ed) Cancer cells in culture. University of Tokyo Press, Tokyo, University Park Press, Baltimore and State College, Pennsylvania, pp 321–334
Katsuta H, Takaoka T (1976) Improved synthetic media suitable for tissue culture of various mammalian cells. In: Prescott DM (ed) Methods in cell biology, vol 14. Academic Press, New York San Francisco London, pp 145–159
Kimura G, Itagaki A, Summers J (1975) Rat cell line 3Y1 and its virogenic polyoma- and SV-40-transformed derivatives. Itl J Cancer 15: 694–706
Niwa A, Yamamoto K, Sorimachi K, Yasumura Y (1980) Continuous culture of Reuber hepatoma cells in serum free arginine-glutamine- and tyrosine-deprived chemmically defined medium. In Vitro 16: 987–993
Rutledge JC, Rudy J (1987) HPLC quantitative amino acid analysis in the clinical laboratories. Am J Clin Pathol 87: 614–618
Shimura H, Ohtsu M, Matsuzaki A (1988) Selective cytotoxicity of phospholipids and diacylglycerols transformed by adenovirus type 12 or its E1A gene. Cancer Res 48: 578–583
Sorimachi K (1992) Inhibition of collagen fiber formation by dextran sulfate in hepatic cells. Biomed Res 13: 75–79
Sorimachi K, Ikeda M, Akimoto K, Niwa A (1995) Rapid determination of dabsylated hydroxyproline from cultured cells by reversed-phase high-performance liquid chromatography. J Chromatogr 664: 435–439
Sorimachi K, Akimoto K, Niwa A, Yasumura Y (1997) Delayed cytocidal effect of lignin derivatives on virally transformed rat fibroblasts. Cancer Detec Preven 21: 111–117
Vendrell J, Aviles FX (1986) Complete amino acid analysis of proteins by dabsyl derivatization and reversed-phase liquid chromatography. J Chromatogr 358: 401–413
Yasumura Y, Niwa A, Yamamoto K (1978) Phenotypic requirement for glutamine of kidney cells and for glutammne and arginine of liver cells in culture. In: Katsuta H (ed) Nutritional requirements of cultured cells. Japan Scientific Societies Press Tokkyo and University Park Press, Baltimore
Zaitsu H, Tanaka H, Mitsudomi T, Matsuzaki A, Ohtsu M, Kumura G (1988) Differences in proliferation properties among sublines of rat 3Y1 fibroblasts transformed by various agents in vitro. Biomed Res 9: 181–197
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okayasu, T., Ikeda, M., Akimoto, K. et al. The amino acid composition of mammalian and bacterial cells. Amino Acids 13, 379–391 (1997). https://doi.org/10.1007/BF01372601
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01372601