Summary
A sample of 202 subjects living in 2 Italian provinces (Ancona and Parma) was tested for YNZ22 polymorphism by the polymerase chain reaction (PCR). After amplification, the phenotypes were separated by agarose gel electrophoresis, stained with ethidium bromide and identified by comparison with a molecular weight marker. No heterogeneity was found between the 2 populations. Alleles, pooled in 4 groups to calculate the Hardy-Weinberg (H-W) equilibrium, showed good accordance between observed and expected values. The power of discrimination (PD) was 0.95 and the chance of exclusion was 0.69. The allele comparison with previous studies on Caucasians showed no significant difference.
Zusammenfassung
Eine Populationsstichprobe von 202 Personen, welche in zwei italienischen Provinzen (Ancona und Parma) leben, wurde mit Hilfe der Polymerase-Kettenreaktion (PCR) auf den Polymorphismus YNZ22 untersucht. Nach Amplifikationen wurden die Phänotypen mit Hilfe der Agarose-Gel-Elektrophorese getrennt und mit Ethidiumbromid angefärbt und durch den Vergleich mit einem Molekulargewichts-Marker identifiziert. Zwischen beiden Populationen bestand keine Heterogenität. Die Allele, welche in 4 Gruppen zusammengefaßt wurden, um das Hardy-Weinberg-Gleichgewicht zu überprüfen, zeigten eine gute Übereinstimmung zwischen beobachteten und erwarteten Werten. Die Diskriminationskraft war 0,95 und die Ausschließungschance war 0,69. Der Allel-Vergleich mit vorhergehenden Untersuchungen an Kaukasiern zeigte keinen signifikanten Unterschied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Batanian JR, Ledbetter SA, Wolff RK, Nakamura Y, White R, Dobyns WB, Ledbetter DH (1990) Rapid diagnosis of Miller-Dieker syndrome and isolated lissencephaly sequence by the polymerase chain reaction. Hum Genet 85:555–559
Budowle B, Baechtel FS (1990) Modifications to improve effectiveness of restriction fragment length polymorphism typing. Appl Theor Electrophor 1:181–187
Deka R, De Croo S, Yu LM, Ferrell RE (1992) Variable number of tandem repeat (VNTR) polymorphism at locus D17S5 (YNZ22) in four ethnically defined human populations. Hum Genet 90:86–90
Fisher RA (1951) Standard calculations for evaluating a blood group system. Heredity 5:95–102
Garber RA, Morris JW (1983) General equations for the average power of exclusion for genetic system of n codominant alleles in one-parent and no-parent cases of disputed parentage. In: Walker RH (ed) Inclusion probabilities in parentage testing. AABB, Arlington, VA, pp 277–280
Horn GT, Richards B, Klinger KW (1989) Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Res 17:2140
Kloosterman A, Vossen R, Wust D, de Leeuw W, Uitterlinden A (1992) Detection of three different VNTR's by DNA-amplification. In: Ritmer C, Schneider PM (eds) Advances in forensic haemogenetics 4, Springer, Berlin Heidelberg, pp 53–56
Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390
Odelberg SJ, Plaetke R, Eldridge JR, Ballard L, O'Connell P, Nakamura Y, Leppert M, Lalouel JM, White R (1989) Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5:915–924
Rand S, Puers C, Skowasch K, Wiegand P, Budowle B, Brinkmann B (1992) Population genetics and forensic efficiency data of 4 AMPFLP's. Int J Leg Med 104:329–333
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
Wolff RK, Nakamura Y, White R (1988) Molecular characterization of a spontaneously generated new allele at a VNTR locus: no exchange of flanking DNA sequence. Genomics 3:347–351
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Buscemi, L., Cucurachi, N., Mencarelli, R. et al. PCR typing of the locus D17S30 (YNZ22 VNTR) in an Italian population sample. Int J Leg Med 106, 200–204 (1994). https://doi.org/10.1007/BF01371337
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01371337